Vol.65, Iss. 2

Dietary inclusion of phytogenic feed additives (PFA) as alternative to in feed antibiotics modulating gut microbial dynamics in broiler chicken

ZUBIA RASHID, ZULFIQAR ALI MIRANI, SITWAT ZEHRA, SYED MUDDASSAR HUSSAIN GILANI, ARIF ALI CHISHTI, ABID AZHAR et al.

Food safety and quality have been a significant and crucial predicament recently. In poultry, development of novel antimicrobial strategies for drug-resistant pathogens is desired. Here, Phytogenic Feed Additives (PFA) were administered in chicken diet to deci…

Monitoring mangroves plantation sites through integration of repeat terrestrial photographs and spaceborne imagery

UROOJ SAEED, SAJID RASHID AHMAD, HAMMAD GILANI, RAB NAWAZ, NAEEM SHAHZAD, IRFAN ASHRAF et al.

This study shows the outcome of realistic steps towards the coastal habitat conservation through mangroves plantation in Keti Bunder (Indus delta), Thatta district, Sindh province, Pakistan. From the visual interpretation of repeat terrestrial photographs and…

Proteolytic activity analysis in germination defective spores of Bacillus

Uqba Mehmood

Proteolytic enzymes are characteristically produced by Bacillus species during sporulation and germination. The hydrolysis of spore proteins to free amino acids, is accomplished by proteases, in first 20 minutes of spore germination. The analysis of Spo mutant…

Hematological and plasma biochemical reference values in Chukar partridge (Alectoris chukar) under captive facilities

ZAHID FAROOQ, IRFAN BABOO, MUHAMMAD WAJID, HAFIZA SADIA, MUHAM

The current study was conducted to determine hematology and blood chemistry of Chukar Partridge kept in captive conditions in Punjab Province of Pakistan. During this study, total 28(10 male, 18 female) blood samples of healthy birds were collected from four d…

Effect of selected organic amendments on germination and yield of Abelmoschus esculentus (L.) moench grown under Chromium stress

ALIA NASEEM, SUMERA IQBAL, KHAJISTA JABEEN

Okra (Abelmoschus esculentus (L.) Moench) was used as test crop in a research trial to assess the impact of organic amendments (compost and farmyard manure) on germination and yield under application of chromium (Cr) stress. The no. of treatments applied was 1…

Bacterial Exopolysaccharides: sources, production and applications

MARIAM ZAHEER, SYED SHAHID IMRAN BUKHARI, MUDDA

Exopolysaccharides (EPSs) are secreted by both eukaryotes (fungi, phytoplankton, and algae) and prokaryotes (archaeobacteria and eubacteria). EPSs produced by bacteria include a broad range of chemical structures and may either be heteropolymeric or homopolyme…

Asthma treatment by practitioners in Lahore-Pakistan

SYED SHAHID IMRAN BUKHARI, ZULFIQAR ALI

The main objective of the study was to investigate the prescription habits of general practitioners dealing with asthma in Lahore. Among 200 practitioners, 166 (83%) responded. One hundred and sixty six asthmatic subjects were selected and given to the practit…

Caffeine concentrations in locally availableenergy drinks of Lahore, Pakistan

MUHAMMAD ASLAM, MISBAH IRSHAD, AMINA ASGHAR, ASAD GULZAR, ZAHRA NOREEN, ABRAR HUSSAIN et al.

Caffeine is a stimulant drug and beneficial to most human being when taken in at low-to-moderate amount, whereas at high amount it may have negative impact on health. Recently the caffeine is being added by companies in energy drinks and other beverages. The a…

Evolution of super-drug resistant microbial strains: mechanisms and strategies for containment

SHEIKH AJAZ RASOOL, MUHAMMAD SALMAN RASOOL, MUNAZZA AJAZ

Super drug resistance (PDR/EDR) in microbial strains has been a continuous phenomenon in nosocomial and miscellaneous infections. The load of these bugs has inflated over worldwide. Microbes evolve such phenomena involving mutational processes, hyper performan…

Optimization of sporulation conditions of Bacillus sublilis as potential probiotics strain

MUHAMMAD SHRAFAT ALI, UZMA HAMEED, IKRAM-UL-HAQ

Probiotics are used as a substitute for antibiotic growth promoters in the chick industry. In the present study, conditions for the sporulation of Bacillus subtilis, a probiotic strain, are optimized. Overnight grown inoculum (1%) was used for the cultivation…

Comparative efficacy of whole blood and hypertonic colloid solution on some blood parameters in resuscitation of acute hemorrhagic shock in Dogs

MUHAMMAD AMJAD ALI, MUMTAZ AHMAD KHAN, WAQAS AHMAD, FAIZ-UL HASSAN, SYED MUHAMMAD RAIHAN DILSHAD, MIAN MUHAMMAD AWAIS et al.

The present study was conducted to compare the efficacy of fluid resuscitation using whole blood and hypertonic colloid fluid in dogs suffering from experimental hypovolemia. Acute hemorrhagic shock was developed in dogs to a touchstone blood pressure of 50 mm…

Bio-screening of Chalcone and Aurone analogues as therapeutic agents

MUHAMMAD MANSHA, QAMAR ALI, UROOJ FATIMA DILAWARI, ABRAR HUSSAIN, MUHAMMAD TARIQ

Chalcones and aurones are compounds having appealing pharmacological profile due to presence of carbonyl system. The bio-screening of 13 compounds was conducted and assessed against S. aureus, C. albicans and E. coli by agar well diffusion method. Chalcones an…

Genetic evaluation of tumor necrosis factor-alpha gene polymorphisms with pre and post term births

UZMA PARVEEN, MASROOR ELLAHI BABAR, NIDA BABAR, TAHIRA MUGHAL, AKHTAR ALI

Tumor necrosis factor alpha is known as multifunctional cytokine component of apoptosis and cell survival. Preterm delivery is a confused prenatal unfavorable occasion. This examination work was planned to associate relationship of G308A and -238 polymorphisms…

Table of Content

In Vitro Antioxidant and Antiglycation properties of extracts from Zingiber officinale, Allium sativum, Caralluma fimbriata and Momordica charantia

Department of Biochemistry and Molecular Biology, University of Gujrat 2Department of Biochemistry, Nawaz Sharif Medical College, University of Gujrat 3Department of Biochemistry, Islam Medical & Dental College, Sialkot   *Corresponding Author: Nadia…

Ultrasonication-assisted extraction, antioxidant activity and α-amylase inhibition potential of Vitexnegundo leaves

MUHAMMAD NADEEM, MUHAMMAD WASEEM MUMTAZ, MUHAMMAD DANISH

The current work describes the ultrasonication assisted extraction followed by comprehensive investigation of antioxidant activity and α-amylase inhibition potential of hydroethanolic leaf extracts of Vitexnegundo. Freeze drying assisted ultrasonicated e…

Isolation and screening of keratinase producing bacteria from soil

MARIAM AHMED, YESRA ARSHAD, HAMID MUKHTAR

Keratinase (E.C 3.4.99.11) is an extracellular protease having efficient ability to degrade keratin found in feathers and hair. It has various applications including leather, textile, pharmaceutical, detergent and animal feed industries. In current study, kera…

Histological responses in Intestine, Kidney and Liver tissues of Labeo rohita during acute and chronic exposure to Pesticide, Chlorpyrifos

ADEEBA SYED, DILAWAR HUSSAIN, UZMA RAFI, SUMAIRA MAZHAR

Aim of the present study was to examine the acute and chronic exposure of pesticide, chlorpyrifos (CPF) to the fresh water fish Labeo rohita. During acute exposure, fish were exposed to different concentrations of CPF ranging from 0, 0.005, 0.006, 0.007, 0.008…

Optimization of extraction process for the recovery of antioxidants from Morus nigra leaves

UMER YOUNAS, SHAHID IQBAL, JAMSHED AKBAR

In current work, effects of different drying procedures as well as of different solvents on extraction of antioxidants from M. nigra leaves have been studied. Initially, samples were dried using different drying procedures (shade, oven and microwave drying) an…

Frequency of Hepatitis C virus genotypes circulating in Gujrat Punjab, Pakistan

MUHAMMAD NAEEM RAZA, KALSOOM SUGHRA, NADIA ZEESHAN

Hepatitis C virus (HCV) infection is primary cause of liver disease that leads toward liver cirrhosis and hepatocellular carcinoma. This study designed to diagnose the prevalence of HCV genotypes circulating in Gujrat, Punjab. Blood samples of 3540 suspected p…

Alterations, complexity and efficient strategies suitable for genetic studies

AFIRA WAQAR, ADNAN RAUF, BUSHRA CHAUDHRY, IKRAM-UL HAQ

Structural design of genetic studies has progressed over the last decade into the most powerful tool for probing the complex human diseases. There are many different approaches for the identification of genetic risk factors of the complex diseases. New pharmac…

Effect of dietary Goji Berry (lyceum barbarumL.) leaf meal on performance, egg quality and egg yolk cholesterol levels of laying Hens

ASUMAN ARSLAN DURU

In this study effects of dietary goji berry (Lycium barbarumL.) leaf (GBL) meal on egg rate, feed intake, egg yield and mass, feed conversion ratio, external and internal egg quality and egg yolk cholesterol level of laying hens were investigated. A total of 5…

Evaluation of antioxidative and antidiabetic potential of piper betle leaf in alloxan induced diabetic albino Rats

HAFIZ MUHAMMAD ARSALAN, SUMMARA TABASSUM, MUHAMMAD SHERAZ YASIN, FAROOQ SALEEM, ZEEMAL SEEMAB AMIN, MUNIB ASHFAQ et al.

Piper betle leaf is deep green heart shaped belongs to family Piperaceae. This plant is used for the treatment of different diseases like diabetes, cancer, inflammatory responses, oxidant injuries etc. in the present study ethanolic extract of Piper betle leaf…

Evaluation of biocontrol strategies and its synergistic interaction permitting the chickpea plant to trigger the appropriate defense responses against Sclerotium rolfsii

AMNA SHOAIB, AROOJ SHEZAD, ARSHAD JAVAID, SUNDUS AKHTAR, ZOIA ARSHAD AWAN

22 Brassicaceae member i.e. Rhaphinus sativus L. and three species of Trichoderma (T. harzianum, T. viride and T. hamatum) were evaluated for their antifungal effect against Sclerotium rolfsii, the causal agent of collar rot disease in chickpea. In vitro, meth…

Fluidic simulation for blood flow in five curved spiral microchannel

MUHAMMAD JAVAID AFZAL, FARAH JAVAID, SHAHZADI TAYYABA, ANEEQA SABAH, MUHAMMAD WASEEM ASHRAF

This study presents ANSYS FLUENT and Fuzzy Logic MATLAB simulation for the study of blood flow in five curved spiral shaped tortuous vein. The dimensions of spiral microchannel used in simulation are 30 mm long and 2 mm in radius. ANSYS simulation has given 0.…