The current study was conducted to determine hematology and blood chemistry of Chukar Partridge kept in captive conditions in Punjab Province of Pakistan. During this study, total 28(10 male, 18 female) blood samples of healthy birds were collected from four different captive facilities under the control of Punjab Wildlife and Parks Department, Government of Punjab, Pakistan. Hematological parameters including Total Red Blood Cell Count, Total White Blood Cell Count, Packed Cell Volume, Hemoglobin, Mean Corpuscular Volume, Mean Corpuscular Hemoglobin, Mean Corpuscular Hemoglobin Concentration, Platelets, Lymphocytes, Monocytes and Neutrophils were determined. The overall red blood cells count, hemoglobin and hematocrit values were 2.00±0.054×106/μL, 11.40±0.350g/dL and 31.30±0.830%, respectively. White blood cells count 220.10±0.857×103/μL and platelets values 5.56±1.431×103/μL were recorded. The sex wise hematological parameter was statistically non-significant (p>0.05) except MPV and P-LCP values which were significantly different (p<0.05). The plasma chemical values including the Urea, Alanine aminotransferase, Aspartate aminotransferase, Creatinine, Total Protein and Albumin were determined. The overall biochemical blood values of Urea 282.06±37.18mg/dL, ALT 13.61±2.597 μL, AST 48.23±28.157 μL, Creatinine 61.04±12.658 mg/dL, Total protein 9.29±1.228 mg/dL and Albumin 2.44±0.108 mg/dL and were recorded. All these parameters between male and female were non-significant (p>0.05). As a pioneer work, these hematological and blood chemistry values may serve as reference range in male and female Chukar Partridge in captivity.