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Utilization of Agro-Industrial Waste for Chitinase Production by Locally Isolated Bacillus subtilis using Response Surface Methodology

Utilization of Agro-Industrial Waste for Chitinase Production by Locally Isolated Bacillus subtilis using Response Surface Methodology

Farah Deeba1, Hafiz Abdullah Shakir1, Muhammad Irfan2, Muhammad Khan1 and Javed Iqbal Qazi1*

1Industrial Biotechnology Lab. Department of Zoology, University of the Punjab, Lahore-54590, Pakistan
2Department of Biotechnology, University of Sargodha, Sargodha 40100, Pakistan.

*      Corresponding author: qazi.zool@pu.edu.pk

Fig. 1.

Conspicuous zone formation on CBM plate by different bacterial isolates.

Fig. 2.

Chitinase production from different isolates in CPB. Different alphabet presents that chitinase production of each isolate was significantly different (P<0.001).

Fig. 3.

Phylogenetic analysis of selected bacterial isolate.

Fig. 4.

Contour plots of chitinase production by Bacillus subtilis showing the interaction of various medium components.

Pakistan Journal of Zoology

February

Vol. 54, Iss. 1, Pages 1-501

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