Trichomonas Gallinae Prevalence and Genetic Characteristics from Racing Pigeons in Thi-Qar Province, Iraq
Trichomonas Gallinae Prevalence and Genetic Characteristics from Racing Pigeons in Thi-Qar Province, Iraq
Ahmed Jaafar Mousa*, Nothaila Rasheed Hamid
ITS1-5.8s rRNA-ITS2 gene amplification in an agarose gel, with M standing for the ladder, 1-2,4-8 for positive results, and 3 for negative results
An alignment of the ITS1-5.8s rRNA-ITS2 sequences from the genomic DNA of 10 parasite samples from Trichomonas gallinae with the proper reference sequences. the NCBI reference sequences are indicated by the notation “ref”.
The Tamura-Nei model and the Maximum Likelihood approach were used to infer the evolutionary history. The tree that has the highest log probability is displayed (-528.91). By automatically applying the Neighbor-Join and BioNJ algorithms to a matrix of pairwise distances calculated using the Tamura-Nei model, and then choosing the topology with the highest log likelihood value, the initial tree(s) for the heuristic search were created. The rate variation model permitted some sites ([+I], 0.00% sites) to be evolutionarily invariable. There were 27 nucleotide sequences in this analysis. Codon positions 1st+2nd+3rd+Noncoding were included. The final dataset contained 374 locations altogether. In MEGA11, evolutionary analyses were carried out (Tamura et al., 2021).
