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Transcriptome Analysis Reveals the Immune Response of Chicken Erythrocytes to Marek’s Disease Virus Infection

Transcriptome Analysis Reveals the Immune Response of Chicken Erythrocytes to Marek’s Disease Virus Infection

Guan-bao Ning1, Sheng Niu1, Yue-jian Li1,2, Xiao-xiao Lu1,3, Shi-xiong Yang1,  Ali-Raza Jahejo1, Ding Zhang1, Wei-fang Hao4, Wen-wei Gao1, Yu-jun Zhao1, Jian-hui Li1, Fang Yan1, Rong-kun Gao1, Yu-hai Bi1,5, Wen-xia Tian1* and Ling-xia Han6*

1College of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China
2Weinan Animal Health Supervision Institute, Weinan 714000, China
3Wenxian Forestry Bureau, Wenxian 454850, China
4Taiyuan Center for Disease Control and Prevention, Taiyuan 030024, China
5CAS Key Laboratory of Pathogenic Microbiology and Immunology, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease, Institute of Microbiology, Center for Influenza Research and Early-warning (CASCIRE), Chinese Academy of Sciences, Beijing 100101, China
6Unit of Laboratory Animal and Comparative Medicine, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
Guan-bao Ning and Sheng Niu contributed equally to this study.

*      Corresponding author:;

Fig. 1.

(A) Numbers of up-regulated and down-regulated DEGs between different cDNA libraries. Blue color represents up-regulated expression of DEGs; Brown color represents down-regulated expression of DEGs. (B) Volcano plot between different cDNA libraries. The log 2 fold represents the mean expression level. Red dots represent up-regulated genes, black dots represent no significant DEGs, the green dots represent down-regulated genes. 14T/14C, the DEGs were generated from the treatment group relative to the control group at 14 dpi; 22T/22C, the DEGs of treatment group relative to the control group at 22 dpi.

Fig. 2.

Gene ontology (GO) categorization of the differentially expressed unigenes (DEG unigenes) in the comparisons (14T/14C and 22T/22C).

Fig. 3.

The heat map of DEGs in three immune-related clusters. X-axis indicated different samples, and the y-axis indicated the gene expression level. Color key ranging from -2 to 2 represents log2 (FPKM+1), indicating low (green) to high (red) expression.

Fig. 4.

Schematic diagrams of cytokine-cytokine receptor interaction. Genes marked in red indicate up-regulated expression in the treatment group relative to the control group, genes marked in green indicate down-regulated expression, genes marked in blue indicate both of upregulated and downregulated expression.

Fig. 5.

Schematic diagrams of the toll-like receptor signaling pathway.

Fig. 6.

Schematic diagrams of the JAK-STAT signaling pathway.

Pakistan Journal of Zoology


Vol. 54, Iss. 5, Pages 2003-2500


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