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The Quality and Proportion of Spermatozoa X and Y in Sexed Frozen Semen Separated with Percoll Density Gradient Centrifugation Method on Friesian Holstein Bull

The Quality and Proportion of Spermatozoa X and Y in Sexed Frozen Semen Separated with Percoll Density Gradient Centrifugation Method on Friesian Holstein Bull

Aulia Puspita Anugra Yekti1, Sri Rahayu3, Gatot Ciptadi2, Trinil Susilawati2*

1Doctoral Student, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, East Java, Indonesia; 2Faculty of Animal Science, Universitas Brawijaya, Malang 65145, East Java, Indonesia; 3Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Malang 65145, East Java, Indonesia.
 
*Correspondence | Trinil Susilawati, Faculty of Animal Science, Universitas Brawijaya, Malang, East Java, Indonesia; Email: [email protected]

Figure 1:

Distribution of response of concentration  (million/straw).

Figure 2:
Distribution of response of total motile spermatozoa (million/straw).
Figure 3:

Observation of capacitation, non capacitation and acrosome status on sperm evaluated by microscope with a magnification of 10 x 40 (A: unsexed semen, B: sexed semen X-bearing sperm), C: sexed semen Y-bearing sperm). White arrows: non capacitation sperm; Red arrows: capacitation sperm; Blue arrows: acrosome reaction.

Equation 1
Equation 2
Equation 3
Equation 4

Advances in Animal and Veterinary Sciences

November

Vol. 12, Iss. 11, pp. 2062-2300

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