Statistical Optimization of Alkaline Protease Production by Newly Isolated Bacillus Strain using Industrial Skin Waste as a Novel Substrate
Najeeb Ullah1*, Muhammad Nadeem2, Malik Mujaddad-ur-Rehman1, Rubina Nelofer2 and Yasir Arfat3
1Department of Microbiology, Abbottabad University of Science and Technology, Abbottabad, Pakistan
2Food and Biotechnology Research Center, PCSIR Labs Complex Lahore 54600, Punjab, Pakistan
3Department of Biotechnology, University of Sargodha, Sargodha, Pakistan
Corresponding author: Najeeb Ullah
Figure 1:
Surface plot representing the interaction effect of substrate and molasses on protease production.
Figure 2:
Surface plot representing the interaction effect o of incubation period and pH on protease production.
Figure 3:
Desirability chart representing the optimum levels for independent variables and predicted protease activity on them.
Figure 4:
The graph between observed and predicted values for the production of protease enzyme.