Short Communication

Seroprevalence of Brucellosis in Occupationally Exposed Humans in Quetta, Pakistan

Tariq Jamil1, Muhammad Saqib2*, Abid Rashid3, Muhammad Hammad Hussain4, Khushal Khan Kasi5, Muhammad Haleem Tayyab2, Muhammad Azam Kakar5, Heinrich Neubauer1 and Mudassar Iqbal6*

1Institute for Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institute, 07743 Jena, Germany; 2Department of Clinical Medicine and Surgery, Faculty of Veterinary Science, University of Agriculture, 38000 Faisalabad, Pakistan; 3Directorate of Medical Sciences, Government College University, 38000 Faisalabad, Pakistan; 4Department of Animal and Veterinary Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Muscat 123, Oman; 5Livestock and Dairy Development Department, Government of Balochistan, 87300 Quetta, Pakistan; 6Department of Pathology, Faculty of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, 63100 Bahawalpur, Pakistan.

Received | April 07, 2023; Accepted | May 27, 2023; Published | June 28, 2023

*Correspondence | Muhammad Saqib and Mudassar Iqbal, Department of Clinical Medicine and Surgery, Faculty of Veterinary Science, University of Agriculture, 38000 Faisalabad, Pakistan; Department of Pathology, Faculty of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, 63100 Bahawalpur, Pakistan; Email: drsaqib_vet@hotmail.com, mudassar.iqbal@iub.edu.pk

Citation | Jamil, T., M. Saqib, A. Rashid, M.H. Hussain, K.K. Kasi, M.H. Tayyab, M.A. Kakar, H. Neubauer and M. Iqbal. 2023. Seroprevalence of brucellosis in occupationally exposed humans in Quetta, Pakistan. Biologia (Lahore), 69(1): 28-31.

DOI | https://dx.doi.org/10.17582/journal.Biologia/2023/69.1.28.31

Keywords | Rose Bengal plate test, Brucellosis, Occupational hazard, Quetta, Pakistan

Copyright: 2023 by the authors. Licensee ResearchersLinks Ltd, England, UK.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

Introduction

Brucellosis is a worldwide zoonotic disease caused by intracellular living Gram-negative bacteria of the genus Brucella (B.). In Pakistan, B. abortus is the main cause of brucellosis in bovines, B. melitensis in small ruminants and B. canis in dogs whereas B. suis has never been reported (Jamil et al., 2021). Infected animals shed the organism in body fluids or birth products e.g., vaginal fluid, milk or fetal membranes which act as source of direct and indirect transmission to humans and other animals. Additionally, consumption of contaminated raw/unpasteurized milk in humans remains a main source of infection (Hakeem and Saeed, 2019). The clinical picture is not pathognomonic but undulant fever, weakness, splenomegaly with occasional reproductive problems are prominent symptoms (Qazilbash and Bari, 1997). Considered as one of the most frequent zoonosis in the world, it infects, an estimated 2.1 million humans worldwide every year (Laine et al., 2023). Brucellosis is one of the priority zoonotic diseases of the National Institute of Health (NIH) in Pakistan and calls have been made to tackle this disease under “One Health” paradigm (CDC, 2017; NIH, 2017). Sporadic cases in humans do occur every year throughout the country but are highly likely to be underreported (NIH, 2022). Balochistan is the largest province (44% by landmass) of the country but studies on human brucellosis are scarce (Ali et al., 2017; Jamil et al., 2021). In this scenario, the purpose of this study was to establish our understanding about the seroprevalence rate of occupationally exposed humans in the district Quetta of Balochistan, Pakistan (Figure 1).

Table 1: Univariable analysis of the risk factors of human brucellosis (n=193) in Quetta, Pakistan.

Materials and Methods

A total of 193 occupationally exposed persons (livestock farm workers, butchers and veterinarians) were recruited conveniently for this study. Biological plausible variables’ data (age, occupation, contact with animals, previous infection history, socioeconomic status and raw milk intake) were noted on a questionnaire proforma. Sera were separated and stored at -20 ℃ until further use. These sera were subjected to Rose Bengal Plate test (RBPT) using Pourquier® Rose Bengal Antigen (IDEXX, Montpellier, France) along with positive and negative control sera provided by the Friedrich-Loeffler-Institut (FLI), Jena, Germany as described by World Organization for Animal Health (OIE, 2022). Briefly, 25µL of each serum were mixed in equal amounts of antigen on a white enamel plate at room temperature and waited for four minutes. A visible agglutination comparable to the positive control serum was considered as positive. Fischer’s exact test was performed on the variables by using SPSS Statistics (IBM Corporation, New York, USA).

Results and Discussion

Four of 193 (2.1%, CI 0.6-5.2) sera were found positive by RBPT in the study. Age >40 years showed higher seroprevalence ratio (5.6%, CI 0.7-18.7) when compared to ≤40 years of age (1.3%, CI 0.2-4.5). Livestock farm workers showed higher seroprevalence (4.1%, CI 0.9-11.5). Seroprevalence (3.6%, CI 0.4-12.5) was found higher in the persons having contact with small ruminants. Poor socioeconomic status showed higher seroprevalence (2.4%, CI 0.3-8.2). Raw milk consumption showed a lower seroprevalence rate (1.9%, CI 0-10.1) than those who didn’t consume raw milk (2.1%, CI 0.4-6.1). A gender-based analysis could not be undertaken as all samples were from male subjects (Table 1). Similarly, multivariate analysis was not considered as all variables showed non-significant association with the seropositivity of the infection.

Although our study could not find a significant association with the factors, based on the previous published data, occupational exposure, duration and age should be considered as risks for brucellosis transmission (Ali et al., 2021). Previously, inadequate knowledge and at least one risky practice have been found in animal workers/owners in different areas of the country (Ali et al., 2016; Arif et al., 2017; Hussain et al., 2021; Tahir et al., 2022). Additionally, contact with the diseased animals and contaminated raw milk consumption could be associated factors in clinical human cases (Hakeem and Saeed, 2019; Saddique et al., 2019). A more variable data for health status would be needed to confirm its association with the disease. Pasteurization of the milk before consumption is recommended as the bacteria is killed when boiled at 62-63 °C for 3 minutes (Murray et al., 1932). Although brucellae respond well to various antimicrobials, culling of the infected animals remains a preferred choice to eliminate the infection. Awareness programs must be implemented.

Acknowledgments

All participants and resource persons are highly thanked for their participation and efforts in this study. Dr. Abdul Rehman from Department of Epidemiology and Public Health, University of Veterinary and Animal Sciences, Lahore, Pakistan is highly thanked for his technical assistance in map creation.

Novelty Statement

This study presents first detailed brucellosis seroprevalence among occupationally exposed individuals in Quetta, Balochistan. Despite being the largest province in Pakistan, prior research on human brucellosis in Balochistan are scarce. Our findings provide a foundation for future research and preventive measures in the region.

Author’s Contribution

MS: Conceptualized and supervised this study.

MAK: Helped collecting the samples.

TJ, MHT and AR: Performed the laboratory work.

KKK and MHH: Performed the analysis.

TJ: Wrote the manuscript.

HN and MI: Reviewed the masnucript.

Ethical statement

The biological specimens were collected as per ethical and standard procedures of the Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad 38000, Pakistan. All participants were prior informed about the possible outcomes of the study and a verbal consent was taken based on the participant’s free will of participation before recruiting in the study.

Conflicts of interest

The authors have declared no conflict of interest.

References

Ali, S., S. Akhter, H. Neubauer, A. Scherag, M. Kesselmeier, F. Melzer, I. Khan, H. El-Adawy, A. Azam, S. Qadeer and Q. Ali. 2016. Brucellosis in pregnant women from Pakistan: An observational study. BMC. Infect. Dis., 16(1): 468. https://doi.org/10.1186/s12879-016-1799-1

Ali, S., A. Akber, M. Shafee, B. Tahira, A. Muhammad and N. Ullah. 2017. Sero-epidemiological study of brucellosis in small ruminants and associated human beings in district Quetta, Balochistan. Pure Appl. Biol., 6: 797-804. http://dx.doi.org/10.19045/bspab.2017.60084

Ali, S., U. Saeed, M. Rizwan, L. Hassan, M.A. Syed, F. Melzer, H. El-Adawy and H. Neubauer. 2021. Serosurvey and risk factors associated with brucella infection in high risk occupations from district Lahore and Kasur of Punjab, Pakistan. Pathogens, 10(5): 620. https://doi.org/10.3390/pathogens10050620

Arif, S., P.C. Thomson, M. Hernandez-Jover, D.M. McGill, H.M. Warriach and J. Heller. 2017. Knowledge, attitudes and practices (KAP) relating to brucellosis in smallholder dairy farmers in two provinces in Pakistan. PLoS One, 12(3): e0173365. https://doi.org/10.1371/journal.pone.0173365

CDC, 2017. One health zoonotic disease prioritization workshop in Pakistan (Islamabad, Pakistan). https://stacks.cdc.gov/view/cdc/84617. Accessed: 19.10.2023

Hakeem, M. and S. Saeed. 2019. Brucellosis: A case report and literature review. J. Postgrad Med. Educ. Res., 53(3): 126-127. https://doi.org/10.5005/jp-journals-10028-1328

Hussain, S., A. Hussain, U.R., Zia, S.M.R. Naqvi, M.Y. Zahoor, M. Bilal, J. Ho and O.A.E. Sparagano. 2021. Knowledge, attitude, and practices associated with brucellosis among livestock owners and its public health impact in Punjab, Pakistan. Biologia, 76: 2921-2929. https://doi.org/10.1007/s11756-021-00765-2

Jamil, T., A.U. Khan, M. Saqib, M.H. Hussain, F. Melzer, A. Rehman, M.Z. Shabbir, M.A. Khan, S. Ali, A. Shahzad, I. Khan, M. Iqbal, Q. Ullah, W. Ahmad, M.K. Mansoor, H. Neubauer and S. Schwarz. 2021. Animal and human brucellosis in Pakistan. Front. Publ. Health, 9: 660508. https://doi.org/10.3389/fpubh.2021.660508

Laine, C.G., V.E. Johnson, H.M. Scott and A.M. Arenas-Gamboa. 2023. Global Estimate of Human Brucellosis Incidence. Emerg. Infect. Dis., 29: 1789-1797. https://doi.org/10.3201/eid2909.230052

Murray, C., S. McNutt and P. Purwin. 1932. The effect of pasteurization upon Brucella melitensis var. suis. J. Dairy Sci., 15(1): 6-13. https://doi.org/10.3168/jds.S0022-0302(32)93382-7

NIH, 2017. Notification of priority diseases in Pakistan. In 1-31/Misc/FEDSD/2017, Health, N.I.o., ed. (Islamabad, Pakistan, Ministry of National Health Services, Regulations and Coordinations), pp. 1-2. https://www.nih.org.pk/wp-content/uploads/2018/11/Notification-of-Priority-Diseases-in-Pakistan.pdf

NIH, 2022. Integrated disease surveillance and response weekly bulletin (Islamabad, Pakistan, National Institue of Health, Islamabad, Pakistan), IDSR-weekly-report-16-2022 and 2011-2022.

OIE Terrestrial Manual, 2022. Brucellosis (Brucella abortus, B. melitensis and B. suis) (Infection with B. abortus, B. melitensis and B. suis). OIE, Paris, France.

Qazilbash, A.A. and A. Bari. 1997. Sero-diagnosis of human brucellosis among TB suspected patients. J. Pak. Med. Assoc., 47(10): 243-246.

Saddique, A., S. Ali, S. Akhter, I. Khan, H. Neubauer, F. Melzer, S.U. Khan, A. Azam and H. El-Adawy. 2019. Acute febrile illness caused by Brucella abortus infection in humans in Pakistan. Int. J. Environ. Res. Publ. Health, 16(21): 4071. https://doi.org/10.3390/ijerph16214071

Tahir, A., S. Naz, M.S. Afzal, R.M.K. Shabbir, S. Ali, N. Shah and H. Ahmed. 2022. Community based assessment on knowledge, attitude and practices (KAP), risk factors and one health perspective of brucellosis in rural and urban settings of Pakistan: A cross-sectional study. J. Hellenic. Vet. Med. Soc., 73(3): 4339-4356. https://doi.org/10.12681/jhvms.26869