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Pseudomonas Species Isolated from Camels: Phenotypic, Genotypic and Antimicrobial Profile

Pseudomonas Species Isolated from Camels: Phenotypic, Genotypic and Antimicrobial Profile

Adel M. Abdelrahman1, Sahar R. Mohamed1, Soliman M. Soliman2, Sherif Marouf3* 

1Bacteriology department, Animal Health Research Institute, Dokki, Giza, 12618, Egypt; 2Department of Medicine and Infectious Disease, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt; 3Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.

*Correspondence | Sherif Abd Elmonam Marouf, Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt; Email: [email protected] 

Figure 1

PCR for P. aeruginosa16S rDNA (956bp). Lane L: 100-1000 bp ladder molecular size (DNA marker). Lane P.: Control positive P. aeruginosa. Lane N.: Control negative. Lanes from 1 to 17: Positive P. aeruginosa 16S rDNA (956bp). 

Figure 2

PCR for mexR gene at (637pbb). Lane L: 100-1000 bp ladder molecular size (DNA marker). Lane P.: Control positive. Lane N.: Control negative. Lanes 1to6,8,13,16,17: Positive for mexR (637bp). 

Figure 3

PCR for blaTEM gene at (516bp). Lane L: 100-1000 bp ladder molecular size (DNA marker). Lane P.: Control positive P. aeruginosa. Lane N.: Control negative. Lanes 1, 2, 4 to 8, 10, 13, 16, 17: Positive for blaTEM gene (516pb). 

Figure 4

PCR for blaSHV gene at (392bp). Lane L: 100-1000 bp ladder molecular size (DNA marker). Lane P.: Control positive. Lane N.: Control negative. Lanes 1, 3, 4, 8, 11, 15, 16, 17: Positive for blaSHV gene(392bp). 

Figure 5

PCR for blaCTX-M gene at (593bp). Lane L: 100-1000 bp ladder molecular size (DNA marker). Lane P.: Control positive. Lane N.: Control negative. Lanes 1, 2, 5, 15, 17: Positive for blaCTX-M gene (593pb).  

Figure 6

PCR for pslA gene at (656bp). Lane L: 100-1000 bp ladder molecular size (DNA marker). Lane P.: Control positive. Lane N.: Control negative. Lanes 2, 3, 13, 15, 17: Positive for pslA gene (656bp). 

Figure 7

PCR for toxA gene at (396bp). Lane L: 100-1000 bp ladder molecular size (DNA marker). Lane P.: Control positive. Lane N.: Control negative. Lanes 3, 10, 13, 16: Positive for toxA gene (396bp).

Figure 8

PCR for exoU gene at (134bp). Lane L: 100-1000 bp ladder molecular size (DNA marker). Lane P.: Control positive. Lane N.: Control negative. Lanes: 3, 7, 8: Positive for exoU gene (134bp). 

Advances in Animal and Veterinary Sciences

December

Vol. 12, Iss. 12, pp. 2301-2563

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