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Protective Effect of Walnut Oil on Alcoholic Liver Disease in Mice

Protective Effect of Walnut Oil on Alcoholic Liver Disease in Mice

Fujun Miao1, Chunlan Shan2, Wei Yang3, Hao Wang3, Shuxiang Geng1 and Delu Ning1*

1Yunnan Academy of Forestry and Grassland, Kunming, 650204, China
2College of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, China
3College of Veterinary Medicine, Yunnan Agricultural University, Kunming, 650201, China.
 
Fujun Miao and Chunlan Shan have equally contributed.
 
* Corresponding author: ningdelu@163.com

Fig. 1.
Effect of walnut oil on liver morphology (200× magnification; HE staining) and ultrastructure (TEM)of ALD mice. N, nucleus; RER, rough endoplasmic reticulum; Mi, mitochondria; LD, lipid droplets.
For details of groups, see Table II.
Fig. 2.
Effects of walnut oil on the expression of F4/80 in liver tissue of mice. A, Identification of F4/80 expression in liver by immunofluorescence (400× magnification; F4/80 staining, red and nuclear staining, blue); B, The F4/80 positive cells rate in liver.
Note: Data are presented as averaged expression levels and standard deviation of three repeats. Different uppercase letters (A, B, C, D) and lowercase letters (a, b, c, d) compared between different groups indicate the significant difference with p < 0.01 and p < 0.05, respectively. The same letters compared between different groups indicate no significant difference (p > 0.05). The same below.
For details of groups, see Table II.
Fig. 3.
The relative expression of TLR4, NF-κB, TNF-α, and IL-1β mRNA in the liver.
For details of groups, see Table II.
Fig. 4.
Effects of walnut oil on the expression of TNF-α in liver tissue of mice. A, TNF-α protein expression in liver by immunohistochemistry (400× magnification) (a, NC; b, ETH; c, ETH + WO; d, WO; e, PC); B, The IOD value of TNF-α protein in liver.
For details of groups, see Table II.

Pakistan Journal of Zoology

August

Vol. 54, Iss. 4, Pages 1501-2001

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