Prevalence of Shiga Toxin Producing Enterohemorrhagic Escherichia coli O157:H7 Isolated from Chicken Meat in Northern Punjab, Pakistan
Balquees Kanwal1*, Syeda Saba Shah1, Syed Waqas Hassan2 and Farzana Shaheen3
1Atta-ur-Rahman School of Applied Biosciences, National University of Sciences and Technology, Pakistan; 2Department of Biosciences, University of Wah, Wah City, Pakistan; 3Department of Chemistry, Allama Iqbal Open University, Islamabad, Pakistan.
*Correspondence | Balquees Kanwal, Atta-ur-Rahman School of Applied Biosciences, National University of Sciences and Technology, Pakistan; Email: balqueeskanwal@gmail.com
Figure 1:
Pie-chart showing the growth on MacConkey agar.
Figure 2:
Growth of lactose fermenter E. coli on MacConkey agar.
Figure 3:
Pie-chart showing growth of lactose fermenting E. coli on sorbitol MacConkey agar.
Figure 4:
Colorless colonies of STEC on sorbitol MacConkey agar.
Figure 5:
Confirmation of Stx-1 (484bp) in 7 samples through PCR (1= marker, 2= control, 3 to 9=chicken samples).
Figure 6:
Confirmation of Stx-2 (779bp) in 7 samples through PCR (1=marker, 2=control, 3 to 9 chicken samples).