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Prevalence of Shiga Toxin Producing Enterohemorrhagic Escherichia coli O157:H7 Isolated from Chicken Meat in Northern Punjab, Pakistan

Prevalence of Shiga Toxin Producing Enterohemorrhagic Escherichia coli O157:H7 Isolated from Chicken Meat in Northern Punjab, Pakistan

Balquees Kanwal1*, Syeda Saba Shah1, Syed Waqas Hassan2 and Farzana Shaheen3

1Atta-ur-Rahman School of Applied Biosciences, National University of Sciences and Technology, Pakistan; 2Department of Biosciences, University of Wah, Wah City, Pakistan; 3Department of Chemistry, Allama Iqbal Open University, Islamabad, Pakistan.

 
*Correspondence | Balquees Kanwal, Atta-ur-Rahman School of Applied Biosciences, National University of Sciences and Technology, Pakistan; Email: [email protected]

Figure 1:

Pie-chart showing the growth on MacConkey agar.

Figure 2:

Growth of lactose fermenter E. coli on MacConkey agar.

Figure 3:

Pie-chart showing growth of lactose fermenting E. coli on sorbitol MacConkey agar.

Figure 4:

Colorless colonies of STEC on sorbitol MacConkey agar.

Figure 5:

Confirmation of Stx-1 (484bp) in 7 samples through PCR (1= marker, 2= control, 3 to 9=chicken samples).

Figure 6:

Confirmation of Stx-2 (779bp) in 7 samples through PCR (1=marker, 2=control, 3 to 9 chicken samples).

Veterinary Sciences: Research and Reviews

June

Vol.10, Iss.1, Pages 1-39

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