Prevalence, Molecular Characterization and Phylogenetic Analysis of Fasciola spp. Based on ITS2 Gene Sequence from Slaughtered Animals in Abattoirs and its public Health in Qualyobia Province, Egypt
Prevalence, Molecular Characterization and Phylogenetic Analysis of Fasciola spp. Based on ITS2 Gene Sequence from Slaughtered Animals in Abattoirs and its public Health in Qualyobia Province, Egypt
Lobna M.A. Salem, Nashwa O. Khalifa, Marwa O. Abd El-Halim*
Fasciola tunnels and fibriosis of liver.
Macroscopic leaf-like Fasciola spp.
Polymerase chain reaction product 300 bp of partial nucleotide sequences of the ribosomal DNA of ITS2 gen. Lane M: DNA ladder (1000 bp). Lane: 1-10 represent Fasciola isolates with DSJf/DSJ3 primer of Fasciola hepatica.
Polymerase chain reaction product 300 bp of partial nucleotide sequences of the ribosomal DNA of ITS2 gen. Lane M: DNA ladder (1000 bp). Lane: 1-10 represent Fasciola isolates with DSJf/DSJ4 primer of Fasciola gigantica.
Fasciola hepatica after stained with Aceto Alum Carmine stain.
Fasciola gigantica after stained with Aceto Alum Carmine stain.
Alignment of ITS2 sequence of F. hepatica (GenBank accession MZ396929.1) and F. gigantica (MN970008.1) deposited in GenBank with F. hepatica and F. gigantica that found in livers of infected animals in Qualyobia Province, Egypt, snaps at 282, 321pb and indel at 310 pb in W7 isolate.
Phylogenetic relationship based on nucleotide sequence of ITS2 gene by phylogeny bootstrap test using neighbor-joining method in MEGA 6.0 software. two isolates cluster in two clades.
