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Phytochemical Screening and In Vitro Antioxidant Activity of Extracts of Ipomoea purpurea Leaves from Iran

Phytochemical Screening and In Vitro Antioxidant Activity of Extracts of Ipomoea purpurea Leaves from Iran

Fatemeh Beheshti1, Maliheh Safavi2, Mohammad Reza Akbari Eidgahi1,3, Parviz Kokhaei4, Mehdi Vazirian5 and  Ali Akbar Shabani1,3*

1Research Center of Biotechnology, Semnan University of Medical Sciences, Semnan, Iran; 2Department of Biotechnology, Iranian Research Organization for Science and Technology, 13353-5111, Tehran, Iran; 3Department of Biotechnology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran; 4Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran; 5Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

 
*Correspondence | Ali Akbar Shabani, Research Center of Biotechnology, Semnan University of Medical Sciences, Semnan, Iran; Email: aashaebani@yahoo.com 

Figure 1:

Antioxidant activity of extracts of I. purpurea leaves assessed by DPPH and FRAP assays. (a) DPPH radical scavenging activity of I. purpurea leaves extracts (ethanol, chloroform, and aqueous) was assessed at different concentrations (20-400 μg/ml) in terms of free radical inhibition percentage. (b) Ferric reducing antioxidant power of extracts of I. purpurea leaves (ethanol, chloroform, and aqueous) assessed at different concentrations (20-400 μg/ml) in terms of FRAP value expressed in μmol Fe2+.

Figure 2:

IC50 values of extracts of I. purpurea leaves required to scavenge the DPPH free radicals. IC50 values of extracts of I. purpurea leaves (ethanol, chloroform, and aqueous) compared with reference (ascorbic acid and BHA). A lower IC50 value indicates higher antioxidant activity.

Equation 1

Pakistan Journal of Zoology

August

Pakistan J. Zool., Vol. 56, Iss. 4, pp. 1501-2000

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