Parasitological and Molecular Survey on Theileriosis of Sheep and Goats and the Related Risk Factors in Musa Pak Shaheed Town, Multan, Pakistan
Muhammad Riaz1*, Zahida Tasawar1, Muhammad Zaka Ullah2 and Zawar Hussain3
Physical Map of Pakistan showing district Multan, the area under study.
Theielria piroplasms in infected RBCs.
Agarose gel electrophoresis of amplified PCR products obtained from Theileria species genomic DNA using Theileria specific primers. Lane M. DNA marker 100-1500 bp; Lane 1. Theileria specific DNA positive control; 4. Negative control (Distilled water); 2.3.5.6.79.10. 11. 13. Theileria species DNA positive sample. Lane .5. 8. 9. 12 Theileria species DNA negative sample.
Agarose gel electrophoresis of amplified PCR products obtained from Theileria ovis and Theileria lestoquardi genomic DNA Lane: M, 100 bp DNA marker; Lane: 1. T. ovis positive control. 3. Negative control (distilled water): 2, 4, 5, 7, 8, Parasite positive blood sample: 6,9, Parasite negative blood sample. Lane: 10. T. lestoquardi positive control. 12 Negative control (distilled water): 11, 13,14,15, 17, 18 Parasite positive blood sample; 16 Parasite negative blood sample.
