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Optimization of Laccase Production Parameters by Aspergillus niger from Different Eco-Logical Sites of Lahore

Optimization of Laccase Production Parameters by Aspergillus niger from Different Eco-Logical Sites of Lahore

Syeda Rubab Zaidi* and Safdar Ali Mirza

Department of Botany, GC University, Lahore, Pakistan

 
Corresponding author: Syeda Rubab Zaidi
rzaidi631@gmail.com, rubabzaidi2009@yahoo.com

Figure 1:

The evolutionary history was inferred by using the maximum likelihood method based on the Tamura 3-parameter model. A discrete Gamma distribution was used to model evolutionary rate differences among sites (5 categories (+G, parameter= 0.2474)). The analysis involved 25 nucleotide sequences. Evolutionary analyses were conducted in MEGA6. The sequence generated in the present study is marked with.

Figure 2:

Optimization of temperature for laccase produce from Aspergillus niger (SAN5).

Figure 3:

Optimization of pH for laccase production from Aspergillus niger (SAN5).

Figure 4:

Optimization of size of inoculum for enzyme production from Aspergilluus niger (SAN5). 

Figure 5:

Effect of incubation time on the production of laccase and fungal mycelial mass of Aspergillus niger (SAN5).

Figure 6:

Carbon source optimization (1%) for production of laccase through Aspergillus niger (SAN5).

Figure 7:

Carbon source optimization (2%) for production of laccase through Aspergillus niger (SAN5).

Figure 8:

Carbon source optimization (3%) for production of laccase through Aspergillus niger (SAN5)

Figure 9:

Wheat straw concentration optimization for laccase production through Aspergillus niger (SAN5).

Figure 10:

Nitrogen source optimization (0.5%) for laccase production through Aspergillus flavus (SAN5).

Figure 11:

Nitrogen source optimization (1%) for production of laccase through Aspergillus niger (SAN5).

Figure 12:

Nitrogen source optimization (1.5%) for production of laccase through Aspergillus niger (SAN5).

Figure 13:

Optimization of urea concentration for laccase production from Aspergillus niger (SAN5).

Figure 14:

Effect of pH on crude enzyme (A), purified enzyme (B) produced by Aspergillus niger.

Figure 15:

Effect of temperature on activity of crude enzyme (A), purified enzyme (B), produced by Aspergillus niger.

Pakistan Journal of Zoology

August

Pakistan J. Zool., Vol. 56, Iss. 4, pp. 1501-2000

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