Molecular Study on Field Evolved Resistance of Red Palm Weevil (Rhynchophorus ferruginous) and its Management through RNAi
Molecular Study on Field Evolved Resistance of Red Palm Weevil (Rhynchophorus ferruginous) and its Management through RNAi
Jam Nazeer Ahmad1,2*,Mujahid Manzoor1, Zubair Aslam3 and Samina Jam Nazeer Ahmad1, 2
Demonstration of the injection of dsRNA in the 5th, 10th instar larvae and adults of R. ferruginous.
P450 expression through PCR in several R. ferruginous larval instars (L1-1st instar, L3- 3rd instar, L5- 5th instar, L7- 7th instar and L10- 10th instar), male (M) and female (F).
P450 down regulation in 10th larval instars (L10) and its knockdown influence via microinjection of dsRNA Conc. (µg/insect).
Expression of P450 proteins by using ant-P450 antibodies.
Expression of P450 gene through qRT-PCR in male, female and larva of R. ferrugineus. (*P<0.05, **P<0.01 and ***P<0.005).
Beta-actin expression in R. ferrugineus larvae (10th instar and Male and Female from left to right) after the application of dsRNA-gfp and dsRNA-P450
Mortality percentage of R. ferrugineus population in response to Pesticide (Cypermethrin) and ds RNA P450 application. SL= “Susceptible larvae”- SM= “Susceptible male”- SF= “Susceptible female”- RL= “Resistant larvae”, RM= “Resistant Male”- RF= “Resistant Female”-, FL= Field larva, FM=Field Male and FF=Field Female (Field population of Sindh province was used. Mortality was observed after 48h of treatment. (*P<0.05, **P<0.01 and ***P<0.00
