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Molecular Identification of Lactic Acid Bacteria from Black Soldier Fly Larvae Reared on Different Substrates

Molecular Identification of Lactic Acid Bacteria from Black Soldier Fly Larvae Reared on Different Substrates

Aulia Nurul Saputri, I Komang Gede Wiryawan, Dilla Mareistia Fassah*, Dewi Apri Astuti 

Department of Nutrition and Feed Technology, Faculty of Animal Science, IPB University, Bogor, 16680, Indonesia.

*Correspondence | Dilla Mareistia Fassah, Department of Nutrition and Feed Technology, Faculty of Animal Science, IPB University, Bogor, 16680, Indonesia; Email: [email protected]  

Figure 1

Morphology identification of LAB isolates. a) Isolate A2. b) Isolate A3. c) Isolate A4. d) Isolate A5. e) Isolate A6. f) Isolate B1. g) Isolate B2. h) Isolate B3. 

Figure 2

Gram staining of LAB isolates. a) Isolate A4. b) Isolate B1 

Figure 3

Hemolysis activity of all isolates. c; Positive control (Staphylococcus aureus). A3; isolate A3. A4; isolate A4. A5; isolate A5. B1; isolate B1. B2; isolate B2. 

Figure 4

Phylogenetic tree of three isolates (A3, A4, and B1).  

Figure 5

Growth curves of three isolates were determined by OD value for 24 hours every 4 hours. (a) Isolate A3. (b) Isolate A4. (c) Isolate B1. 

Advances in Animal and Veterinary Sciences

November

Vol. 12, Iss. 11, pp. 2062-2300

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