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Molecular Detection of Anaplasma marginale and Theileria annulata in Buffaloes by Using Duplex PCR from Sheikhupura, Jhang, and Rawalpindi Districts of Punjab, Pakistan

Molecular Detection of Anaplasma marginale and Theileria annulata in Buffaloes by Using Duplex PCR from Sheikhupura, Jhang, and Rawalpindi Districts of Punjab, Pakistan

Saba Mehnaz1*, Farhan Ahmad Atif2, Rao Zahid Abbas1, Muhammad Kasib Khan1 and Muhammad Saqib3

1Department of Parasitology, Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan.
2Medicine Section, Department of Clinical Sciences, College of Veterinary and Animal Sciences, Jhang (Sub-Campus), University of Veterinary and Animal Sciences, Lahore, Pakistan.
3Department of Clinical Medicine and Surgery, Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan.
 
*   Corresponding author: raouaf@hotmail.com, sabamehnaz2012@gmail.com

Fig. 1.

District wise prevalence of Anaplasma marginale and Theileria annulate in study districts.

Fig. 2.

Phylogenetic analysis of Anaplasma marginale based on msp1β gene. Our revealed sequences are indicated with Pak-3* and Pak-4*.

Fig. 3.

Phylogenetic analysis of Theileria annulate based on cytochrome b gene. Our revealed sequences are indicated with Pak-2*.

Pakistan Journal of Zoology

August

Pakistan J. Zool., Vol. 56, Iss. 4, pp. 1501-2000

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