Molecular Characterization and Secondary Metabolite Profiling of the Actinomycetes Strains Active against Various MDR Bacterial Pathogens
Molecular Characterization and Secondary Metabolite Profiling of the Actinomycetes Strains Active against Various MDR Bacterial Pathogens
Nimra Naseer, Adeela Fatima and Imran Sajid*
Pure cultures ofstreptomycetesstrains on GYM agar grown at 28oC for 5 to 7 days. A, NWMD-5; B, NWMD-12; C, NWMD-18.
Microscopic appearance of streptomycetes strains. A, NWMD-1; B, NWMD-15.
Antimicrobial activity of ofstreptomycetes strains (A) Streptomycetes strain NWMD-7 by cross streak method. On left side gram negative is streaked and on right side gram positive is streaked perpendicular to streptomycetesgrowth (B) Streptomycetes strain NWMD-13, NWMD-14, NWMD-15 and NWMD-16 against MRSA-1 by agar plug method. (C) Supernatant of streptomycetes strain NWMD-7, NWMD-8, NWMD-9, NWMD-10, NWMD-11, and NWMD-12 against MRSA-1 by well diffusion method (D) Methanolic extracts of streptomycetesstrains NWMD-7, NWMD-8, NWMD-9, NWMD-10, NWMD-11and NWMD-12 against Klebsiella by well diffusion method (E) Methanolic extracts of streptomycetes strains NWMD-15, NWMD-16, NWMD-17, NWMD-18 and NWMD-19 against MRSA by well diffusion method (F) Methanolic extracts of streptomycetes strains NWMD-6, NWMD-7, NWMD-8, NWMD-9 and NWMD-10 against MRSA by well diffusion method.
Chemical screening using TLC. A, TLC plate under UV at short wavelength (254 nm) of strain NWMD-1 to NWMD-8; B, TLC plate under UV at long wavelength (366 nm)of strain NWMD-1 to NWMD-8; C, TLC plate after treatment with Anisaldehye / H2SO4of strain ND-1 to ND-8; D, TLC plate after treatment with Ehrlisch reagent of strain NWMD-1 to NWMD-8; E, TLC plate under UV at short wavelength (254 nm) of strain NWMD-9 to NWMD-15; F, TLC plate under UV at long wavelength (366 nm)of strain NWMD-9 to NWMD-15; G, TLC plate after treatment with Anisaldehye / H2SO4of strain NWMD-9 to NWMD-15; H, TLC plate after treatment with Ehrlisch reagent of strain NWMD-1 to NWMD-8.
HPLC-UV chromatogram of Streptomyces strains. A, NWMD-7; B, NWMD-10; C, NWMD-12.
