Modulation of Pentylenetetrazol Induced Neuronal Circuit Epilepsy by p2y Purinoreceptor 3 (p2y3) in Sprague Dawley Rats
Modulation of Pentylenetetrazol Induced Neuronal Circuit Epilepsy by p2y Purinoreceptor 3 (p2y3) in Sprague Dawley Rats
Zhongjiang Niu1, Periyannan Velu2, Annamalai Vijayalakshmi2*
The P2Y3 antagonist NF45 has an effect on the mean kindling score of PTZ-kindling animals. (A) Seizure stage for each group. (B) Days of stimulation for each group at various phases. (C) Each group’s average kindling score.
The P2Y3 antagonist NF45 has an effect on the mean kindling score of PTZ-kindling animals. (A) Seizure stage for each group. (B) Days of stimulation for each group at various phases. Group I, No treatment; Group II, infused with artificial cerebrospinal fluid; Group III, administered with NF 45; Group IV, administered with PTZ; Group V, administered with PTZ+30nm NF45; Group VI, administered with PTZ+60nm NF45; Group VII, administered with PTZ+90nM NF45; Group VIII, α-β- methylene-ATP+90nM NF45+PTZ.
The P2Y3 antagonist NF45 has an effect on the anxiety behaviour of PTZ-kindling animals. (A) The amount of time spent in open and closed arms. (B) Frequency in the open and closed arm. For details of groups, see Figure 2.
The effect of the P2Y3 antagonist NF45 on the ability of PTZ-kindling animals to discriminate. (A) The exploration time was identical in all groups in test 1 (T1), where both objects were similar or familiar (DO1 and DO2). (B) NF45 improved the separation of FO1 and NO significantly. For details of groups, see Figure 2.
Effect of the P2Y3 antagonist NF45 on learning and memory of PTZ-kindling animals, as assessed on Morris water maze. (A) PTZ impaired learning of rats and NF45 mitigated this damage in PTZ-kindling animals. (B, C) In a dose-dependent manner, NF45 raised the TSTQ and number of crossings in the platform area of PTZ-kindling animals on day 5. For details of groups, see Figure 2.
Effect of the P2Y3 antagonist NF110 on PTZ-kindling animals’ hippocampal IL-1β and TNF-α levels. (A) In PTZ-kindling animals, NF45 significantly reduced hippocampal IL-1β. (B) In PTZ-kindling rats, NF110 dramatically lowered hippocampus TNF-α levels. For details of groups, see Figure 2.
Effect of the P2Y3 antagonist NF45 on hippocampal mitochondrial complex I, II, and IV levels of PTZ-kindling animals. NF45 significantly increased the hippocampal mitochondrial complex I (A), II (B), and IV (C) levels of PTZ-kindling animals.
Effect of the P2Y3 antagonist NF45 on learning and memory of PTZ-kindling animals, as assessed on Morris water maze. In a dose-dependent manner, NF45 raised the TSTQ and number of crossings in the platform area of PTZ-kindling animals on day 5. For details of groups, see Figure 2.
