Microplastics: Their Effects on Amphibians and Reptiles-A Review

Dong-Min Hou and Ding-Qi Rao*

Kunming Institute of Zoology, Chinese Academy of Sciences, No. 17 Longxin Road, Panlong District, Kunming 650201, China

ABSTRACT

Although microplastics provide much convenience to humans, they also pose a threat to the ecological environment on which humans depend. Microplastics persist in the environment and spread throughout the planet through the atmosphere, ocean currents, wind and animals, and it of different shapes and sizes can directly or indirectly affect organisms and pose health risks to animals and humans. Therefore, the size and types of microplastics and their effects on different organs of different types of animals were reviewed in this paper. However, we found that the health risks posed by microplastics to amphibians and reptiles remain unknown. Then, we reviewed the effects of microplastics on amphibians and reptiles, and most previous studies have focused on turtles and tadpoles; by comparison, few studies have been conducted on snakes, lizards and crocodiles. Although the extinction of some species has increased awareness of the need for conservation, there are still many gaps in research on the effects of microplastics on amphibians and reptiles. More research on the effects of microplastics on amphibians and reptiles is needed to to learn more about the potential effects and direct ongoing future conservation efforts. Finally, some constructive countermeasures are proposed to promote microplastics research in amphibians and reptiles.

Article Information

Received 20 August 2022

Revised 24 December 2022

Accepted 05 January 2022

Available online 02 June 2022

(early access)

Published 20 September 2022

Authors’ Contribution

D-MH and D-QR conceived the study and participated in its design, coordination and drafted the manuscript. Both the authors read and approved the final manuscript.

Key words

Plastic pollution, Ecological hazard, Microplastics

DOI: https://dx.doi.org/10.17582/journal.pjz/20210820080823

* Corresponding author: [email protected]

0030-9923/2022/0006-2931 $ 9.00/0

Copyright 2022 by the authors. Licensee Zoological Society of Pakistan.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

Introduction

Plastics are high molecular weight compounds that are produced either by addition polymerization or polycondensation reactions with monomers extracted from coal, oil and natural gas (Derraik, 2002). Since the development of synthetic polymers in the 1950s, the production and use of plastic products have increased (Geyer et al., 2017). In 2019, approximately 368 million tons of plastic were produced (Plastics Europe, 2020). Plastic products are widely used for their light weight, high strength, durability, low cost, high ductility and stable properties (Cressey, 2016). Plastic products are currently used in various products, including medical equipment, building supplies, personal care products, household products and toys (Hu et al., 2019).

Microplastics have been found in diverse parts of the globe including marine environments, freshwater habitats and soil, and they can persist in the environment for long periods (Wu et al., 2019). In our daily life, we have often found discarded pesticide plastic bottles (Fig. 1A) and plastic films (Fig. 1B); with the sun’s ultraviolet radiation and the wind, they gradually decompose into smaller pieces of plastic, which can eventually mix into the soil. The harmful and toxicological effects of microplastics can vary because of their different shapes and properties, and the persistence of microplastics in the environment stems from their small particle size and weak photodegradation ability (Sruthy and Ramasamy, 2017; Zhang et al., 2018). A variety of additives in waste plastic products may be released in the process of recycling and natural aging, and most of these additives are harmful (Hermabessiere et al., 2017; Hahladakis et al., 2018). Microplastics can react with other pollutants to produce more complex secondary pollutants with higher compound toxicity (Sighicelli et al., 2018). In addition to greatly harming the ecological environment, microplastics affect human health as they are passed through the food chain (Su et al., 2016). Microplastics are almost everywhere in the environment (Fig. 2).

Because of rapid human population growth, the intensification of human activities and frequent natural disasters, the ecological environment has deteriorated and continues to deteriorate, the habitat of many species has become fragmented or even disappeared and populations have sharply decreased; this has contributed to a rapid decline in global biodiversity and accelerated the rate of species extinction (Dirzo et al., 2014; Seddon et al., 2016; Kremen and Merenlender, 2018). According to Hoffmann et al. (2010), approximately 20% of vertebrates (25% of mammals, 13% of birds, 22% of reptiles and 41% of amphibians) are threatened by extinction, and the outlook for amphibians and reptiles was not optimistic (Urban, 2015). Amphibians and reptiles are key components of ecosystems, and habitat protection is considered key for amphibian and reptile conservation (Cortes-Gómez et al., 2015). Consequently, several studies have examined how various environmental variables affect amphibians and reptiles, such as the water environment, soil environment, threats, temperature and humidity (Acevedo-Charry and Aide, 2019; de la Vega-Pérez et al., 2019; Navas et al., 2021). Species diversity has also been a major focus of amphibian and reptile conservation research. Several studies examining species composition, elevational patterns, floristic distribution characteristics, threatened species and new species have aided species diversity conservation (Buckley and Beebee, 2004; Hilje and Aide, 2012; Popgeorgiev et al., 2014).

 

The objectives of this review were to (1) outline the size and types of microplastics and their effects on different organs of different types of animals and (2) clarify the effects of microplastics on amphibians and reptiles and their conservation. Several research directions relating to the physical and biochemical effects of microplastics on amphibians and reptiles that merit future study are also discussed.

 

Status of RESEARCH on MICROPLASTICS

Microplastics of different particle sizes

The concept of microplastics was first proposed in 2004, and microplastics were defined as plastic debris and particles with diameters less than 5 mm (Thompson et al., 2004). Qi et al. (2020) further divided microplastics into small microplastics (< 1 mm), medium microplastics (1–3 mm), large microplastics (3–5 mm), nanoplastics (1–1000 μm) and picoplastics (< 1 μm) (Andrady, 2011; Andrés Rodríguez-Seijo, 2018; Gesamp, 2015; Horton et al., 2017; Liu et al., 2018; Rillig, 2012; Qi et al., 2020). In a comprehensive study on microplastic pollution, 1167 fish samples were collected in southern Germany. The results show that more than 95% of the particles may be less than 40 μm. Beyond the detection range of most other micro plastic investigations today. Moreover, the smaller particle size of microplastics in this study may increase the prevalence of microplastics in fish to 100%; And 70% of the particles are less than 5 μm. Therefore, it is more suitable to transfer to the organization, which is of key significance for fish health and consumer contact (Roch et al., 2019). The same problem has been found in other studies, for example, study on microplastics in the Asian clam (Corbicula fluminea) in Taihu Lake, China, the result shown that the size of microplastics ranged from 0.021-4.83 mm, and microplastics in the range of 0.25-1 mm were dominant (Su et al., 2018). This showed that in addition to the larger plastic products such as plastic bottles and plastic bags that are usually visible to the naked eye, more and more plastic products have begun to decompose into smaller particles, and can even escape the general detection means.

The effects of microplastics of different shapes and sizes have been studied, and this work has shown that plastic products including plastic bottles, plastic bags and plastic debris can become entangled with animals, suffocate them or damage their organs following ingestion. Vasaruchapong and Chanhome (2013) found a wild-captured female Monocellate Cobra (Naja kaouthia) in an abnormal posture that had difficulty moving because of swelling in the middle third of the body. Surgery later revealed that the swelling was caused by a plastic bottle and a piece of cloth in the gastrointestinal tract. After removing these items, the snake fully recovered. However, a wild King Cobra (Ophiophagus hannah) did not fare as well after ingesting an opaque plastic bag with unknown contents. The snake’s physical condition rapidly deteriorated, which was reflected by the substantial weight loss and large area of wrinkled skin. Over time, the surrounding muscle tissue atrophied, and the nerve arch became more prominent (Strine et al., 2014).

Studies of plastics of different sizes viz. millimeters, microns and nanometers have also been conducted. Dong et al. (2018) evaluated the size-dependent migration and retention of micron-sized plastic spheres (2.0, 1.5, 0.8, 0.6, 0.4 and 0.1 µm) in marine sandy environments with different salinities. They found that the aggregation of 0.6, 0.4 and 0.1 μm microplastics weakens their fluidity. At 17.5, 3.5 and 0 PSU, the effect of salinity on the transport of microplastics depends on the size of microplastics. The mechanism by which cell transport is promoted under high ionic strength varies among plastic particles of different sizes; the adsorption of 0.02 μm microplastic particles on the surface of cells and the obstruction caused by suspended plastic particles can promote cell transport (He et al., 2018). Plastic particles are confirmed carriers of pollutants, and microplastic particles of different sizes have different Deposition rates in the marine and soil environment (Brennecke et al., 2016; Cai et al., 2016; Virsek et al., 2017). In addition, the capacity of microplastics of the same particle size to adsorb pollutants and their effects on animals vary at different concentrations (Velzeboer et al., 2014; Lu et al., 2018a; Kim and An, 2019).

Microplastics of different material types

Plastics can be divided into several different types based on their chemical composition, including polyethylene (PE), polyvinyl chloride (PVC), polypropylene (PP), polystyrene (PS), polyamide (PA), polycarbonate (PC), polyethylene terephthalate (PET), phthalate acid esters (PAEs), polytetrafluoroethylene (PTFE), polymethyl methacrylate (PMMA) and acrylonitrile butadiene styrene copolymers (ABS) (Takada, 2006; Teuten et al., 2009; Kasirajan and Ngouajio, 2012; Steinmetz et al., 2016; Alimi et al., 2017; Koelmans et al., 2019; Gao et al., 2021; Serrano-Ruiz et al., 2021).

Plastic products of different materials vary in the risks they pose to human and animal health. Rochman et al. (2013) pointed out that monomers and other components of PVC, PS, PC and polyurethane may cause cancer and affect the hormones of organisms according to laboratory tests (vom Saal and Hughes, 2005; Teuten et al., 2009; Lithner et al., 2011). These materials are generally made of toxic materials that are difficult to recycle. However, the effects of some of the plastic products are more benign, such as PE (Rochman et al., 2013). The continuous exposure of fish to high concentrations of PVC may have negative effects on fish physiology (Espinosa et al., 2017). The effects of PS nanoparticles of different sizes (50 and 100 nm) and types (plain PS, carboxyl-modified and amine-modified) on organisms can vary (Lundqvist et al., 2008). In addition, surface functional groups appear to affect the affinity of organic matter and Ca2+ to nanoplastics (Song et al., 2019). Based on aggregation kinetics experiments and Derjaguine-Landaue-Verweye-Overbeek theoretical calculations, nano PS has high stability in aquatic environments (Mao et al., 2020). PS, PE, PP, PVC and other microplastic particles can easily adsorb pollutant particles, and the adsorption capacity of microplastics increases as the pollutant concentration increases; the deposition of pollutants in the water environment and soil environment can harm the ecological environment (Hirai et al., 2011; Velzeboer et al., 2014; Wang et al., 2015).

Because of the wide application of plastic products, the content of the same type of plastic product can vary among locations, and the concentration of PAEs is higher in more developed areas (Kong et al., 2012). With the spread of urbanization, the content of phthalate esters in the soil of suburban wasteland has increased; furthermore, the use of plastic film in non-urban areas has led to a gradual increase in the content of PAEs in soil (Kasirajan and Ngouajio, 2012; Kong et al., 2012; Steinmetz et al., 2016). Although He et al. (2015) proposed the use of microbial degradation, phytoremediation and bioavailability by adsorption to remediate PAE-contaminated soil, remediation remains difficult because we lack a sound understanding of the mechanisms underlying the wide distribution of phthalate esters in soil. In addition to affecting soil quality and pollutant accumulation, microplastics (e.g., PE and PP) can affect soil microbial diversity and bacterial community structure (Cheng et al., 2021).

Effects of microplastics on different organisms

The effects of plastics vary among animals, and plastics can directly or indirectly induce chemical toxicity, including growth inhibition, reductions in fecundity, alterations of enzyme activity and increases in mortality (Sharma and Chatterjee, 2017; Wright and Kelly, 2017; Barboza et al., 2018; Jin et al., 2018, 2019; Lu et al., 2018a). Microplastics research on terrestrial vertebrates has mainly focused on rodents. Deng et al. (2017) showed that the distribution of microplastics in animal tissues depends on their size; specifically, large particles (20 μm diameter) tend to be distributed in all tissues, whereas small particles (5 μm diameter) tend to be concentrated in the intestines. In addition, metabolites involved in lipid metabolism increased, and choline decreased, in the serum of mice exposed to microplastics (Wei et al., 2008; Wright et al., 2013). Microplastics research on terrestrial invertebrates has mainly focused on the earthworm (Lumbricus terrestris). Earthworms produce more mucus when they ingest substances that are not rich in fresh organic matter (Trigo et al., 1999). The increase in the microplastics concentration (high doses) in garbage may stimulate earthworms to produce more intestinal mucus, the growth and weight of earthworms are reduced (Lwanga et al., 2016). Microplastics may also lead to changes in the energy distribution of another worm (Eisenia andrei) (Rodriguez-Seijo et al., 2017).

Aquatic animals are one of the most well-studied groups of animals in the field of microplastics. In environments with high concentrations of microplastics, the swimming ability of fish is weakened, predation efficiency is decreased, the oral cavity is vulnerable to damage and mortality increases because of inflammation (Sharma and Chatterjee, 2017; Wright and Kelly, 2017; Barboza et al., 2018; Jin et al., 2018, 2019; Lu et al., 2018b). Aside from the concentration of microplastics, the exposure time to microplastics can substantially affect fish: the health status of fish decreases as the exposure time increases (Li et al., 2020a). PE and PET have been documented in shellfish, which are commonly consumed by humans, and these microplastics can transfer nutrients in the food chain (Naji et al., 2018). Détrée and Gallardo-Escárate (2018) exposed the edible Mediterranean mussel (Mytilus galloprovincialis) to different concentrations of microplastics and found that homeostasis was altered, and stress and immune-related proteins were produced, which resulted in a decrease in the energy required for growth. When mussels were exposed to an environment without microplastics, apoptosis was activated, and immune receptors and stress-related proteins were up-regulated. Furthermore, microplastics also induce intestinal macrobiotic imbalance and specific bacterial changes, which will provide new insights into the potential mechanism of intestinal toxicity caused by microplastics (Jin et al., 2018; Qiao et al., 2019).

Several studies have been conducted on the toxic reactions of birds, insects and even nematodes to microplastics. Reynolds and Ryan (2018) explored the potential environmental threat of microplastics in African freshwater systems by testing and analyzing 283 fecal samples and 408 feather brushings. They found that 5% of fecal samples and 10% of feather samples contained microplastic fibers. Galleria mellonella, Tenebrio molitor and Plodia interpunctella can degrade PE and PS microplastics, which may be related to their intestinal bacterial flora (Yang et al., 2014; Xu and Zhang, 2018; Tang et al., 2020). The ability of microplastic particles to induce death and reproductive dysfunction in Caenorhabditis elegans, decrease the intestinal calcium level and increase the expression of oxidative stress genes depends on the size of the microplastic particles (Lei et al., 2018a).

Effects of microplastics on different organs

Many studies have examined the toxicity of microplastics to different organs, including those of the respiratory system, digestive system, nervous system, immune system and reproductive system.

Respiratory system: Examination of 114 human lung specimens revealed fibers in 99 cases (87%), including plastic fibers (Pauly et al., 1998). PS nanoparticles were detected in the lung, testis, spleen, and heart of rats, which indicates that PS nanoparticles can circulate throughout the body (Walczak et al., 2015).

Digestive system: Digestive system has mainly focused on the gastrointestinal tract, liver. Exposure to microplastics leads to decreased intestinal mucus secretion, decreased intestinal microflora richness and liver lipid disorder in mice (Jani et al., 1990; Lu et al., 2016, 2018a; Deng et al., 2017, 2020; Lei et al., 2018a; Jin et al., 2019; Stock et al., 2019; Li et al., 2020b). Rainieri et al (2018) evaluated the differential gene expression of some biomarker genes selected by zebrafish in liver, intestine and brain. In addition, perfluorinated compounds in the liver, brain, muscle tissue and intestine of some selected samples were quantified. The addition of microplastics containing adsorbed pollutants produced the most obvious effect, especially on the liver (Rainieri, et al., 2018).

Nervous system: Schirinzi et al. (2017) exposed human brain cells and epithelial cells to an environment polluted by PE and PS in vitro and then explored chemical toxicity at the cellular level by measuring oxidative stress and cell viability. They found that oxidative stress is one of the cytotoxic mechanisms at the cellular level. When rats were exposed to PS nanoplastics, subtle and transient effects on neurobehavior were observed (Rafiee et al., 2018). The results of Lei et al. (2018b) indicate that 1 μm PS particles have the most substantial effect on the survival, development and motor-related neurons of nematodes. Plastic nanoparticles can move up the food chain, enter the brain of top consumers and affect their behavior (Mattsson et al., 2017).

Immune system: Microplastic particles can cause vascular occlusion in animals and humans (Jones et al., 2003) and blood coagulation (Churg and Brauer, 2000); they may also be toxic to blood cells (Canesi et al., 2015). Furthermore, microplastics can affect the immune response and immunosuppression of organisms (Saravia et al., 2014; Canesi et al., 2015; Détrée and Gallardo-Escárate, 2018).

Reproductive system: Microplastics may induce reproductive toxicity through oxidative stress or the activation of signaling pathways (Xie et al., 2020). Microplastics can result in abnormal sperm quality and testosterone levels in male mice (Hou et al., 2021; Jin et al., 2021); exposure of pregnant female mice to PS microplastics increases the risk of metabolic disorders in their offspring (Luo et al., 2019).

EFFECTS OF MICROPLASTICS ON AMPHIBIANS AND REPTILES

Effects of microplastics on amphibians

Most studies of the effects of plastics on amphibians have focused on the effects of microplastics on tadpoles. Boyero et al. (2020) proposed three reasons for the need to explore the effect of microplastics on amphibians: (1) the causes of the global amphibian decline are manifold (Blaustein et al., 2011), and microplastics may interact with other causes of their decline (Horton et al., 2017); (2) tadpoles are important primary consumers in freshwater ecosystems that may consume microplastics, and this might affect primary production, nutrient cycling and other processes (Whiles et al., 2013); and (3) the accumulation of microplastics in amphibians may lead to the transfer of pollutants through trophic levels and across ecosystem boundaries (Larsen et al., 2016).

To date, research on the effects of microplastics on amphibians has mainly focused on three aspects (Table I). The first is the toxicity of bisphenol A (BPA) to tadpoles. BPA is a synthetic organic compound used for manufacturing polycarbonate plastics and epoxy resins (Bhandari et al., 2015). High concentrations of BPA have been shown to affect the sexual and physical development of amphibians (Wolkowicz et al., 2016; Arancio et al., 2019). Although the experimental study of high concentrations of BPA does not replicate the natural concentrations of BPA in the ecological environment (aquatic organisms: 0–9340 ng/g; surface water: 0–63640 ng/L, Wu and Seebacher, 2020), research on this aspect is still of value because it provides basic data such as concentration thresholds for the toxic damage of microplastics to amphibians. The second focus is on the toxic effects caused by direct contact or ingestion. After a short period of exposure to microplastics, the movement ability, anti-anxiety behavior and anti-predator responses of Physalaemus cuvieri tadpoles decreases (Araújo and Malafaia, 2020); additionally, the accumulation of microplastics in tadpoles causes pathological changes in liver tissue (Araújo et al., 2019). The third focus is on the detection of microplastics in amphibians in the natural environment. Since awareness of the need for natural environments to be protected has increased, amphibians have been shown to be highly sensitive to pollutants (Buck et al., 2012) and habitat changes (Ficetola et al., 2015). Several studies (e.g., Araújo et al., 2021) have begun to explore the effects of microplastics on nutrient transfer in tadpoles.

Effects of microplastics on reptiles

Habitat degradation and overexploitation have made reptiles some of the most endangered animals on the planet (Rhodin et al., 2018; Stanford et al., 2020; Clause et al., 2021). Kolenda et al. (2021) recently evaluated 503 animal samples trapped in abandoned containers around the world, and reptiles (15.3%) were the second most common animals after mammals (78.5%); the proportion of animals trapped in glass or plastic cans and beverage bottles was 48.9%. The effect of microplastics on turtles has been a major focus of research (Table II). Reptiles can become easily entangled with plastics and can ingest large pieces of plastic, such as plastic bottles, bags or straws, which can result in physical injury, including asphyxia or organ damage. Well-known examples include turtles with an obstructed nasopharynx following the inhalation of plastic pipes, which increases the difficulty of breathing, as well as turtles entangled by plastic nets, which limits their mobility (Gregory, 2009; Casale et al., 2010; Jensen et al., 2013; Barreiros and Raykov, 2014; Vegter et al., 2014; Nelms et al., 2016). Entanglement may cause long-term pain, a gradual deterioration in their health, reduce their foraging ability, drowning or death by starvation (Barreiros and Raykov, 2014; Nelms et al., 2016). Turtles may also be trapped in plastic debris from land sources (Chatto et al., 1995).

Snakes are one of the main groups of reptiles. Snakes have often been documented to be entangled by plastic bags (Sindha et al., 2020) and swallow plastic bottles (Vasaruchapong and Chanhome, 2013), bags (Strine et al., 2014; Deshmukh et al., 2017) and bottle caps (Lettoof and Orton, 2020), which can negatively affect their health and even result in death (Table II). Udyawer et al. (2013) reported that a sea snake (Hydrophis elegans) on the northeast coast of Queensland, Australia died because it was

 

Table I. A number of studies on the effects of (micro)plastics on amphibians.

Mainly aspects	Authors	Research subjects	Main contents
Toxicity of bisphenol A in tadpoles	Bhandari et al., 2015	Aquatic wildlife species including fish, amphibians, aquatic reptiles, aquatic mammals, etc.	Clarify the effects of the environmental estrogenic contaminants bisphenol A and 17α-ethinyl estradiol on sexual development and adult behaviors in aquatic wildlife species.
	Tamschick et al., 2016	Xenopus laevis, European tree frog (Hyla arborea) and European green toad (Bufo viridis)	Bisphenol A diversely affects amphibians with different evolutionary history, sex determination systems and larval ecologies.
	Wolkowicz et al., 2016	Rhinella arenarum (Fam. Bufonidae)	Industrial wastewaters containing bisphenol A, diglycidyl ether, as well as its migration substances and metabolites, represent potential sources of aquatic contamination, which may disrupt populations of amphibian.
	Gao et al., 2018	Chinese giant salamander (Andrias davidianus)	Bisphenol A treatment reduced bodyweight and ERα expression in the gonads in male larvae.
	Arancio et al., 2019	X. laevis	Early exposure to Bisphenol A, Bisphenol AF, or di-n-butyl phthalate has significant, concentration dependent effects on early cleavage, neural development, and embryo survival.
	Wu et al., 2020	Seven taxonomic groups (162 species) across 31 countries	Clarify the effect of the plastic pollutant bisphenol A on the biology of aquatic organisms.
	Zhu et al., 2020	X. laevis	Bisphenol A and bisphenol F at environmentally relevant concentrations can activate Notch signaling and subsequently disrupt intestinal development in vertebrates.
	Niu et al., 2020	X. laevis	Comparison on acute toxicity and stress induction of bisphenol A with its substitutes to Xenopus laevis. Bisphenol A is less harmful than bisphenol AF, and is close to bisphenol A.
Toxicity caused by direct contact or ingestion	Mathieu-Denoncourt et al., 2015	Western clawed frogs (Silurana tropicalis)	Monomethyl phthalate is unlikely to threaten amphibian populations as only concentrations four orders of magnitude higher than the reported environmental concentrations altered the animal physiology.
	Gardner et al., 2016	X. laevis	Developing Xenopus laevis exposed to diethyl, di-n-propyl, and di-n-butyl showed similar malformations that also occurred at lower concentrations with increasing alkyl chain length.
	Hu et al., 2016	Xenopus tropicalis	Microspheres were likely to be ingested and egested relatively fast by tadpoles. Aquatic vertebrate organisms might ingest more microplastics if the abundance of microplastics continues to increase while the available food becomes less.
	De Felice et al., 2018	X. laevis	Polystyrene microplastics can be ingested by tadpoles, but they did not alter X. laevis development and swimming behavior at least during early-life stages, also at high, unrealistic concentrations.
	Araújo et al., 2019	Physalaemus cuvieri	Polyethylene microplastic accumulates in tadpole liver and induces histopathotoxicity in P. cuvieri. Polyethylene microplastic bioaccumulation in tadpoles’ liver was correlated to different histopathological changes.
	Boyero et al., 2020	Midwife toad (Alytes obstetricans)	Microplastics can be an important source of stress for amphibians in addition to other pollutants, climate change, habitat loss or chytrid infections, and that amphibians can be a major transfer path for microplastics from freshwater to terrestrial ecosystems.
			Table continues on next page................
Mainly aspects	Authors	Research subjects	Main contents
	Araújo et al., 2020	Physalaemus cuvieri	Polyethylene microplastic accumulation in P. cuvieri tadpoles can affect important biological aspects such as locomotion ability, anxiogenic behavior, and antipredator response deficit in anurans exposed to potential predators.
	Buss et al., 2021	Wood frog (Rana sylvatica)	Polyester microplastic fibers can augment host–parasite interactions, but only at high concentrations.
Detection of (micro)plastics	Hu et al., 2018	Microhyla ornata, Rana limnochari, and Pelophylax nigromaculatus and/or Bufo gargarizans	The dominant shape and polymer of microplastic in water and tadpole samples were polyester fibers, and polypropylene fibers and fragments were dominant in sediment samples. Tadpole length was positively correlated to the number of microplastics detected.
	Iannella et al., 2020	Italian crested newt (Triturus carnifex)	Livestock pressure directly influences Triturus carnifex diet and highlight that the emerging issue of plastics is a threat even in remote high-altitude environments.
	Karaoğlu and Gül, 2020	Pelophylax ridibundus and Rana macrocnemis	In tadpoles, polyethylene terephthalate, nylon, and polyacrylic were the dominant microplastics.
	Kolenda et al., 2020	Bufo bufo, Rana temporaria, Pelophylax esculentus, Pelobates fuscus and Hyla arborea	IR-ATR analysis revealed that particles were of anthropogenic origin and included nylon, polyurethane, polyisoprene and 1,2 polybutadiene.

 

Table II. A number of studies on the effects of (micro)plastics on reptiles.

Classify	Mainly aspects	Authors	Research subjects	Main contents
Biochemical effects	Ingestion	Ng et al., 2016	Chelonia mydas	Plastics and other foreign materials were found in the stomach contents of 2 of the 8 individuals sampled.
		Pham et al., 2017	Caretta caretta	The increasing quantity of plastic debris in the North Atlantic pose a significant risk for loggerhead populations that are already under pressure of other anthropogenic threats such as fishing activities.
		Duncan et al., 2019	mydas, C. caretta, Lepidochelys kempii, Dermochelys coriacea, Natator depressus, Eretmochelys imbricata, Lepidochelys olivacea	The results showed that synthetic particles being isolated from species occupying different trophic levels suggest the possibility of multiple ingestion pathways.
		Clukey et al., 2017	L. olivacea, C. mydas, C. caretta, D. coriacea	Comparing the four species, authors found that pelagic juvenile green turtles ate the greatest amounts of plastic and proportionally more sheets and line.
		White et al., 2018	C. caretta, C. mydas, E. imbricata	This study of ingested micronizing plastic in stranded post-hatchling sea turtles correlates with the ratio of production levels of plastic for disposable consumer markets.
		Jung et al., 2018a	L. olivacea, C. mydas, C. caretta	Low-density polyethylene and polypropylene, some of the most produced and least recycled polymers worldwide, account for the largest percentage of plastic eaten by sea turtles in the Central Pacific.
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Classify	Mainly aspects	Authors	Research subjects	Main contents
		Wilcox et al., 2018	C. mydas, E. imbricata, C. caretta, N. depressus, L. olivacea, two unidentified dead turtles	The results provide the critical link between recent estimates of plastic ingestion and the population effects of this environmental threat.
		Velez-Rubio et al., 2018	C. mydas	The results detected a negative correlation between the presence of plastics debris and turtle's size.
		Domènech et al., 2019	C. caretta	The composition of marine debris (occurrence and amounts of different categories) was similar to that found in other studies for the western Mediterranean and their amounts seem not to be an important threat to turtle survival in the region.
		Eastman et al., 2020	C. caretta	This report demonstrates that plastic ingestion is a critical issue for marine turtles from the earliest stages of life.
		Digka et al., 2020	C. caretta	Results indicated a variation in plastic ingestion amongst life stages of the loggerhead specimens.
		Machovsky-Capuska et al., 2020	C. mydas	The realized nutritional niche from estuarine turtles was subject to the debris density in the environment, lack of benthic food resources available and the surface foraging behavior, likely preventing them from reaching their nutritional goals and resulting in lower fitness.
	Effects on organs	Di Renzo et al., 2021	C. caretta	Microplastics and additives surely impact the health status of turtles that showed gastrointestinal impairment and an important level of contamination in tissues.
		Savoca et al., 2018	D. coriacea , C. caretta	The total concentration of all analyzed phthalates, showed high values in all tissues.
		Colferai et al., 2017	C. mydas	The patterns of anthropogenic marine debris distribution along the gastrointestinal tract and their relationship with obstructions and faecalomas in 62 green turtles (Chelonia mydas) that died during rehabilitation in southern Brazil were determined.
		Galoppo et al., 2017	Caiman latirostris	The alterations in the caiman female reproductive tract exposed to Bisphenol A highlight the importance of preserving aquatic environments from plastic pollution.
		Banaee et al., 2020	Emys orbicularis	Microplastics intake induced notable alterations in blood biochemical parameters of Emys orbicularis.
	Detection method	Jung et al., 2018b	L. olivacea, C. mydas, C. caretta	Of 828 ingested plastics pieces from 50 Pacific sea turtles, 96% were identified by attenuated total reflectance Fourier transform infrared spectroscopy (ATR FT-IR) as High-density and low-density polyethylene, unknown PE, polypropylene (PP), PE and PP mixtures, polystyrene, polyvinyl chloride, and nylon.
		Caron et al., 2018	C. mydas	The authors developed a microplastic extraction protocol for examining green turtle (Chelonia mydas) chyme, which is multifarious in nature, by modifying and combining pre-established methods used to separate microplastics from organic matter and sediments.
		Gonzalez-Jauregui et al., 2019	Crocodilians	The method used during the experiment consists of 1) immobilization of the crocodile; 2) extraction of microplastics from stomach contents obtained through stomach flushing; 3) separation, identification and quantification of recovered microplastic fragments using microscopy and FTIR.
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Classify	Mainly aspects	Authors	Research subjects	Main contents
		Marn et al., 2020	C. caretta	Overcomes the difficulties by modelling individual ontogeny under reduced energy intake and expenditure caused by debris ingestion. The predicted ontogeny is combined with a population dynamics model to identify ecological breakpoints: cessation of reproduction or negative population growth.
Physical effects	Habitat environmental monitoring	do Sul et al., 2011	Sea turtle	The results showed that the majority (~52-94%) was plastic debris regardless of the sampling approach, considered sources or season of sampling in Brazil (Costa dos Coqueiros, Bahia State).
		Beckwith and Fuentes, 2018	C. caretta	The results indicate that microplastic accumulation on nesting sites for the Northern Gulf of Mexico may be of great concern, and could negatively affect the incubating environment for marine turtles.
		Gündoğdua et al., 2019	C. mydas	Macroplastic pollution can cause negative effects, especially entanglement and entrapment, on green sea turtle females and hatchlings.
	In vivo removal or degradability	Müllera et al., 2012	C. mydas, C. caretta	The gastrointestinal fluids of the herbivorous Green turtle showed an increased capacity to break down the biodegradable polymer relative to the carnivorous Loggerhead, but at a much lower rate than digestion of natural vegetative matter.
		Andrades et al., 2019	C. mydas	Opportunistic scavenging behavior, an adaptive behavior in most marine ecosystems, may now pose a threat to a variety of marine animals due to the current widespread plastic pollution found in oceans.
	Odors and colors	Pfaller et al., 2020	C. caretta	Understanding the mechanisms that underlie the attractiveness of marine plastics is therefore critical for optimizing mitigation efforts to protect wildlife and ecosystems threatened by the ever-rising levels of marine plastic debris.
		Santos et al., 2016	C. mydas	Floating darker debris were ingested over the proportions found in the environment and lighter debris under the proportions in green turtle.
	Entanglement or ingestion	Vasaruchapong and Chanhome, 2013	Naja kaouthia	A plastic bottle and a piece of cloth causes a wild-captured female monocellate cobra (Naja kaouthia) had abnormal posture and move with difficulty as consequences of swelling at the middle third of the body.
		Udyawer et al., 2013	Hydrophis elegans	A ceramic washer had constricted the body and over time caused extensive damage to the underlying tissues.
		Strine et al., 2014	Ophiophagus hannah	Improper disposal of food and plastic waste can be a threat to snakes, highlighting the need to maintain a waste-free environment, especially in areas inhabited by vulnerable species.
		Deshmukh et al., 2017	Bungarus caeruleus	The consumed plastic bag halts the digestion and restricts movement so that the snake becomes an easy meal for another predator or scavenger, which could in turn suffer the same consequences.
		Lettoof and Orton, 2020	Notechis scutatus	The organs around the bottle cap appeared to be heavily damaged, it is unlikely the snake could have passed any waste from this blockage.
		Sindha et al., 2020	Python molurus	Incidents involve the death of snakes entangled in fishing nets, which traps the pythons until they drown.
		Barreiros and Raykov, 2014	C. caretta	Plastic debris and discarded/lost nylon fishing gear are part of a serious pollution problem affecting all the world’s oceans.

 

trapped by a ceramic ring. The ceramic ring prevented food from entering the stomach and intestines of the snake. Plastic rings of similar shapes and sizes are likely widespread in the environment. We speculate that these plastic rings may cause the death of other snakes. Thus, microplastics in the environment can potential to affect reptiles. There is a need for more research to characterize the types of injury induced by microplastics on reptiles and the factors affecting the likelihood of injury.

CONCLUSIONS AND PERSPECTIVES

Plastic pollution is now considered a major factor responsible for the global decline in biodiversity and is a major threat to the functioning of ecosystems and human health (Gall and Thompson, 2015). Although many countries have implemented new measures on the use of plastics, such as using paper materials to make straw instead of plastic straw and the plastic boxes made of environmentally friendly materials, a large amount of plastic waste is still discharged into the natural environment, which affects natural habitats and the animals that occupy them. Although the effects of microplastics have received attention from various researchers, research on the effects of microplastics or macroplastics on amphibians and reptiles is still lacking. With the continual increase in the production and use of plastic products, there is a pressing need to document the effect of microplastics on amphibians and reptiles to aid their conservation. In addition to research on the effect of microplastics on amphibians and reptiles, green products need to be increasingly used to protect the environment for the well-being of both humans and animals.

The following research directions relating to the physical and biochemical effects of microplastics on amphibians and reptiles would be particularly fruitful.

Physical effects

Investigation of the effects of different shapes, sizes and types of microplastics on amphibians and reptiles: Different amphibians and reptiles have different feeding habits. Generally, snakes can swallow larger food, while frogs use their tongues to prey on insects. Moreover, the breathing mode of frogs is also special. Tadpole larvae breathe with gills, and adults can breathe through lungs and skin. Therefore, microplastics of different sizes and types on land or water have different effects on different amphibious and reptile species.

The distribution of microplastics in different areas, habitats, altitudes and other environments where amphibians and reptiles are found and its effect on amphibians and reptiles: Generally, with the change of environment such as altitude and regional type, the range of human activities will change accordingly, and the concentration of microplastics produced by human activities will also change. The distribution of amphibians and reptiles in different environments is also different, so the impact of microplastics on amphibian and reptile populations is also different in different environments.

Investigation on the effect of entanglement of plastic debris products on amphibians and reptiles: In order to facilitate human production and life, some plastic products are made into grids, such as fishing nets, shading nets, etc. These grid shaped plastic products are not only a barrier for amphibians and reptiles to move on land, but also affect the swimming trajectory in water.

Biochemical effects

Study of the toxic effects of microplastics on amphibian and reptile larvae and adults: If microplastics are absorbed by amphibians and reptiles, it is difficult to discharge smoothly as excreta. When they exist in animals for a long time, they will release the chemical toxicity of microplastics, thus affecting animal health.

Toxic effects of microplastics on different organs of amphibians and reptiles: The size and function of different organs of amphibians and reptiles are different. There are also different ways of inhalation and discharge of microplastics. At the same time, they also have different enrichment degrees in different organs. Therefore, it is very necessary to study this part.

Study of the effect of plastic concentration and exposure time on amphibians and reptiles: We speculate that different microplastics concentrations and different times have different toxic effects on amphibians and reptiles, and the differences of effects need a lot of scientific research.

ACKNOWLEDGMENTS

This work was supported by the National Natural Science Foundation of China (31772424; 31970404). We thank Liwen Bianji (Edanz) (www.liwenbianji.cn/) for editing the English text of a draft of this manuscript.

Statement of conflict of interest

The authors have declared no conflict of interest.

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