Mass Culturing of Mycetophagous Nematode Aphelenchus avenae (Nematoda: Aphelenchidae) in vitro System by Feeding on Pathogenic Fungus
Salma Javed and Samreen Khan*
National Nematological Research Centre, University of Karachi, Karachi-75270, Pakistan.
*Correspondence | Samreen Khan, National Nematological Research Centre, University of Karachi, Karachi-75270, Pakistan; Email:
[email protected]
Figure 1:
A and B. Isolation of Aspergillus niger from banana leaves; C. Pure culture of Fusarium oxysporum; D. Pure culture of Aspergillus niger; E and F. Interaction between fungus and nematodes; G. Fungal spores; H. Bunch of nematodes along with eggs; I. Mass culture of A. avenae after grew on F. oxysporum; J. Various embryogenetic stages of A. avenae.
Figure 2:
Increasing in population of A. avenae on F. oxysporum with no growth on Aspergillus niger during three weeks incubation period.
Figure 3:
Exponential growth of A. avenae on F. oxysporum grew on PDA after three weeks incubation period.
Figure 4:
A. Pure culture of F. oxysporum in test tube; B-C. Change in appearance and lessening of F. oxysporum after inoculation of A. avenae; D-E. Consuming fungus as well PDA medium by A. avenae.
Figure 5:
Culturing of A. avenae in a pot using brinjal plant.