Lipase Production from Aspergillus niger Used as a Biocatalyst for Transformation of Used Cooking Oil into Biodiesel
Ahsan Abbas Abro1, Imrana Khushk1, Abdul Nabi Jatt2, Chaudhary Haider Ali3, Abdul Sattar Qureshi1*, Farah Naz Talpur4, Safia Bano1
1Institute of Biotechnology and Genetic Engineering, University of Sindh, Jamshoro (76080), Sindh, Pakistan.
2Institute of Microbiology, University of Sindh, Jamshoro (76080), Sindh, Pakistan.
3Department of Chemical, Polymer and Composite Materials Engineering, University of Engineering and Technology, KSK Campus, Lahore, Pakistan
4Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro (76080), Sindh, Pakistan.
Corresponding Author: Abdul Sattar Qureshi
Figure 1:
Step-wise experimental scheme.
Figure 2:
Characterization of crude lipase (a) reaction time; (b) lipase concentration; (c) substrate concentration.
Figure 3:
Characterization of crude lipase (a) pH and pH stability of lipase; (b) temperature and thermostability; (c) effect of metal ions on lipase activity.
Figure 4:
Biodiesel production from waste cooking oil using crude lipase produced by A. niger. (a) reaction time; (b) reaction temperature; (c) methanol to oil molar ratio; (d) enzyme dosage; (e) agitation speed.