Isolation and Molecular Identification of Clostridium perfringens Type D in Goats in District Peshawar
Sidra Shehzadi, Sher Bahadar Khan*, Umar Sadique and Saqib Nawaz
College of Veterinary Sciences, The University of Agriculture, Peshawar, Pakistan.
*Correspondence | Sher Bahadar Khan, College of Veterinary Sciences, The University of Agriculture, Peshawar, Pakistan; Email: sbvetdr@yahoo.com
Figure 1:
Tube A and B showed liquefaction due to hydrolysis of gelatin while tube C showed solidification.
Figure 2:
Positive sample of C. perfringens Type D (tube 1-5) show positive indole by appearing red rose layer to the top while tube C are negative control.
Figure 3:
C. perfringens type D on PCR, Lane 1: 1kb gene marker. Lane 2, 3 showing positive results of epsilon Toxin Gene with 206 bp clear bands. Lane 4: Show negative control while lane 5 shows positive control.