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Isolation and Molecular Identification of Clostridium perfringens Type D in Goats in District Peshawar

Sidra Shehzadi, Sher Bahadar Khan*, Umar Sadique and Saqib Nawaz

College of Veterinary Sciences, The University of Agriculture, Peshawar, Pakistan.

 
*Correspondence | Sher Bahadar Khan, College of Veterinary Sciences, The University of Agriculture, Peshawar, Pakistan; Email: sbvetdr@yahoo.com

Figure 1:

Tube A and B showed liquefaction due to hydrolysis of gelatin while tube C showed solidification.

Figure 2:

Positive sample of C. perfringens Type D (tube 1-5) show positive indole by appearing red rose layer to the top while tube C are negative control.

Figure 3:
C. perfringens type D on PCR, Lane 1: 1kb gene marker. Lane 2, 3 showing positive results of epsilon Toxin Gene with 206 bp clear bands. Lane 4: Show negative control while lane 5 shows positive control.

Sarhad Journal of Agriculture

March

Sarhad Journal of Agriculture, Vol.40, Iss. 1, Pages 01-262

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