Isolation and Identification of Antifungal Bacteria from Rhizosphere of Citrus Field
Isolation and Identification of Antifungal Bacteria from Rhizosphere of Citrus Field
Amir Abdullah Khan and Muhammad Irfan*
Map of Sargodha, Pakistan sampling sites.
(A) Control Schizophyllum sp. B2A. (B) Streak plate method showing antifungal activity of Bacillus subtilis 2i against Schizophyllum sp. B2A. (C) Well diffusion method, showing antifungal activity against Schizophyllum sp. B2A.
Relationship between isolates Bacillus subtilis 2i and several other strains based on their 16SrRNA gene sequences through neighbor- joining Phylogenetic tree.
Relationship between Schizophyllum sp. B2A and several other strains based on their 18SrRNA gene sequences through neighbor-joining Phylogenetic tree.
Effect of temperature on antifungal substance production.
Effect of pH on antifungal substance production.
Effect of inoculum size on antifungal substance production.
Effect of incubation period on antifungal substance production.
Effect of temperature on stability of fermented broth.
Effect of pH on stability of fermented broth.
Effect of UV on stability of fermented broth.
