Study was conducted at Dire Dawa, Haramaya University Research Station in naturally root-knot nematode
infested soil to determine the influence of poultry litter and rapeseed cake application against root-knot nematode
infestation in tomato var. Mraglobe. Five levels of poultry litter (control (0), 5, 10, 15 and 20 ton/ha) and three
levels of rapeseed cake (control (0), 100, 200 kg/ha) was applied in experimental plot three weeks before
transplanting the seedlings. The experiment laid in RCBD factorial arrangement. Application of poultry litre
except 5 ton/ha significantly reduced eggs per egg-mass, root galling and final population of root-knot nematode
at p < 0.001. Rapeseed cake at 200 kg/ha significantly reduced final population and eggs per egg-mass compared
to control treatment at p < 0.05. Applications of poultry litter at 5 ton/ha to 15 ton/ha in combination with
rapeseed cake at 200 kg/ha remarkably suppressed root-knot nematode infestation.