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Influence of Varying Dietary Cation-Anion Difference on Blood Metabolites of Holstein Dairy Cows

Influence of Varying Dietary Cation-Anion Difference on Blood Metabolites of Holstein Dairy Cows

Hazem E. M. Hassanien1*, Awad M. M. Mahmoud2, Elsayed M. Abdel-Raouf1, Nabil M. Eweedah1 and Midhat. N. Nassif3

1Department of Animal Production, Faculty of Agriculture, Kafrelsheikh University, Kafr El Sheikh, Egypt.
2Animal Production Research Institute, Agriculture Research Center, Ministry of Agriculture, Giza, Egypt.
3Department of Internal Medicine, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El Sheikh, Egypt.
 
*      Corresponding author: hazem_hassanien@yahoo.com

Fig. 1.

Urine pH (Means±SE) for cows fed prepartum DCAD (Tr1) 0.0, -100, -180 (BC1, BC2, BC3) and postpartum DCAD (Tr2) +250 and +350 (AC1, AC2) showing effect of interaction (BC1AC1, BC1AC2, BC2AC1, BC2AC2, BC3AC1, BC3AC2), there is effect of prepartum DCAD (Tr1) between means for cows fed 0.0, -100, -180 (7.5, 7.02 and 5.82), P < 0.005, there is effect of time sample -2, -7 ( 6.45 and 6.68) and -14 (7.21) P < 0.05 and the interaction Tr1 x T, P = 0.247. postpartum there is effect of treatment Tr2 x T time of sample at 14, 7 urine pH (8.15 and 8.17), 2 days after calving pH (7.38), P> 0.05, also there is interaction after calving Tr1 x T time of sample p. 0.05 but there is no interaction after calving from Tr1 x Tr2 x T, P = 0.690.

Fig. 2.

Simple linear regression and negative linear correlation between urine pH and iCa (R = -0.74, R2 = 0.548) urine pH = (14.2 + -1.61*iCa) for cows fed prepartum (Tr1) DCAD (0.0, -100, -180) there is negative linear relationship urine pH and iCa p < 0.05.

Fig. 4.

Means serum iCa concentrations for cows fed prepartum and effect of Tr1 DCAD 0.0, -100, -180 (BC1, BC2, BC3. P < 0.001) and effect of postpartum Tr2 DCAD +250 and +350 (B1A1, B1A2, B2A1, B2A2, B3A1, B3A2. P = 0.511), day P < 0.001 and interaction Tr1 x Tr2 x day P = 0.510, during experimental period.

Fig. 5.

Simple linear regression and positive correlation between tCa and iCa (R = 0.84, R2 = 0.712 P < 0.001) tCa = 3.2 + 1.23*iCa for cows fed prepartum (Tr1) DCAD (0.0, -100, -180) there is positive linear relationship tCa and iCa p < 0.05.

Fig. 3.

Means of tCa concentrations for cows fed prepartum and effect of (Tr1) DCAD 0.0, -100, -180 (BC1, BC2, BC3. P < 0.001) and effect of postpartum (Tr2) DCAD +250 and +350 (B1A1, B1A2, B2A1, B2A2, B3A1, B3A2. P = 0.511), day P < 0.001 and interaction Tr1 x Tr2 x day P = 0.086, during experimental period.

Pakistan Journal of Zoology

October

Vol. 54, Iss. 5, Pages 2003-2500

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