Improving the Phytosanitary of Fig Plants Infected with Fig Latent Virus by Nanochitosan and Biomagic
Hanaa H.A. Gomaa1*, Dalia Y.A. Amin1, Mona A. Ismail1, Basma Hamdy2, Khaled A. El-Dougdoug3
1Department of Botany and Microbiology, Faculty of Science, Suez Canal University, Ismailia, Egypt; 2Regional Center for Mycology, Al-Azhar University, Cairo, Egypt; 3Department of Microbiology, Faculty of Agriculture, Ain Shams University, Cairo, Egypt.
*Correspondence | Hanaa H.A. Gomaa, Department of Botany and Microbiology, Faculty of Science, Suez Canal University, Ismailia, Egypt;
Email: [email protected]
Figure 1:
Fig plants showing healthy and infected showed a wide range of foliar discoloration and malformation symptoms, Vein feathering, Vein banding, Leaf deformation, Chlorosis, Mosaic. Fig trees treated with ChNPs (T-ChNPs), fig trees treated with Biomagic (T-bio) and fig trees treated with ChNPs and Biomagic (T-ChNPs and Bio) correction of the effects caused by the Fig latent virus infection.
Figure 2:
Agarose gel electrograph 1.5% showing PCR products at expected size of 520 bp for CP gene of FLV detected infected fig plant cv. Sultani. C = infected fig plant treated with ChNPs; B= infected fig plant treated with biomagic; CB= infected fig plant treated with ChNPs +Bio magic and V= Infected fig plant.
Figure 3:
Photograph showing increased leaf area and shoot length of FLV infected fig plants and foliar with ChNPs and biomagic compared with infected fig plants.
Figure 4:
Transverse sections of the middle part of the leaf lamina, blade and petiole of healthy and FLV infected untreated and treated fig plant with ChNPs and or biomagic (X400).
Figure 5:
Ultra-micrograph section in mesophyll tissue of healthy infected and treated fig leaf ChNPs and biomagic showing: (A) Impact cells and (B) Magnified nucleus (N) showing compact nucleus in healthy cell and deformed nucleus in infected cell. CW: cell wall; Ch: chloroplast; V: Virus; Vs: vesicles.