Histopathological and Molecular Characterization of Heterophyids in Fish and Attitude Towards Parasitic Zoonosis among Residents in Kafrelsheikh Governorate, Egypt
Histopathological and Molecular Characterization of Heterophyids in Fish and Attitude Towards Parasitic Zoonosis among Residents in Kafrelsheikh Governorate, Egypt
Samah Abou Asa1, Omnia Tag2 and Walid Elmonir2*
PCR amplification of 18S rDNA and COI genes of EMC detected in examined tilapia and mullet fish samples. A, PCR results for 18S rDNA gene. B, PCR results for COI gene. Lanes 1-10: tilapia fish samples. Lanes 11-32: Mullet fish samples. M: 100 bp DNA marker. P, Control positive; N, Control negative.
Histopathological findings of EMC infection in muscles of tilapia and mullet fish samples. A, Mullet muscle showing EMC embedded in the intermuscular fat (arrow). B, Nile tilapia muscle showing EMC (arrowhead) between the muscle bundles with few mononuclear cells infiltration (arrow). C, Mullet muscle showing marked degenerative changes of the muscles associated with mononuclear cells infiltration (arrow) and melanophores(arrowhead) with foal areas of muscular hyalinization (thick arrow). D, Mullet muscle showing marked necrotic changes of the muscle bundles with lysis of myofibers (arrowheads). E, Mullet muscle showing atrophy of muscle bundles (arrow heads) associated with destruction and loss of muscle bundles irregularities (arrow). F, Tilapia muscle showing atrophy of muscle bundles (arrowhead) accompanied with mononuclear cells infiltration (arrow). Stain H and E, x200, scale bar 50μm.
