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Haemolysin, Catalase and Hydrogen Sulphide Production as Unique Phenotypic Virulence Determinants, Biofilm Formation and Defense against Antibiotics among Mycoplasma arginini and Mycoplasma ovipneumoniae Isolated from Pneumonic Lungs of Domestic Sheep (Ovis aries)

Haemolysin, Catalase and Hydrogen Sulphide Production as Unique Phenotypic Virulence Determinants, Biofilm Formation and Defense against Antibiotics among Mycoplasma arginini and Mycoplasma ovipneumoniae Isolated from Pneumonic Lungs of Domestic Sheep (Ovis aries)

Mona M. Osman1, Kamelia M. Osman2, Manal Abu Elmakarem Mohamed1, Mahmoud E. Hashad2, Jeeser Alves Almeida3, Alaa Saad4, Octavio Luiz Franco5,6, Heba N. Deif 2* 

1Mycoplasma Department, Animal Health Research Institute, Giza, ARC; 2Microbiology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt; 3Graduate Program in Health and Development in Midwest Region, Faculty of Medicine, Federal University of Mato Grosso do Sul, Brazil; 4Food Hygiene Department, Animal Health Research Institute, Giza, Egypt; 5Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília-DF, Brazil; 6S-inova Biotech, Programa de Pós-Graduação em Biotecnologia, Universidade Católica Dom Bosco, Campo Grande-MS, Brazil.

*Correspondence | Heba N. Deif, Microbiology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt; Email: [email protected] 

Figure 1

Clustering heatmap of virulence traits phenotypes by lungs and nasal swab. 

Figure 2

Clustering heatmap of antimicrobial resistance by lungs and nasal swab. 

Figure 3

Maximum Likelihood phylogenetic tree of 16S ribosomal RNA gene, partial sequence of sheep Mycoplasma arginini (five isolates) and M. ovipneumoniae (four isolates) in comparison with other representative strains of M. arginini and M. ovipneumoniae available on NCBI. 

Figure 4

Maximum Likelihood phylogenetic tree of 16S ribosomal RNA gene, partial sequence of goat M. arginini (four strains) and sheep M. arginini (five strains), Cattle M. arginini (one strain), Camel M. arginini (four strains) in comparison with human M. arginini (two strains) on Genbank, and other representative strains of M. arginini available on NCBI. Camel ━, Sheep ●, Goat □, Human ━ and cattle □ isolates. 

Supplementary Figure S1

Sequence results submitted to the Genbank. 

Advances in Animal and Veterinary Sciences

November

Vol. 12, Iss. 11, pp. 2062-2300

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