Genetic Diversity and Genotyping of Canine Parvovirus Type 2 by Using the Full-Length Vp2 Gene in North Iraq
Genetic Diversity and Genotyping of Canine Parvovirus Type 2 by Using the Full-Length Vp2 Gene in North Iraq
Zaniar A. Abas1, Mohammed Omer Baba Sheikh2,5*, Hardi N. Aziz3, Omed I. Abid4
Figure 1
Specific fragment of the VP2 gene (583bp) was amplified with primer 555f/55r. Lane M DNA marker (100bp), Lane C+, positive control, Lane C-, Negative control; Lane 1-5 positive sample
Figure 2
Amino acid sequence alignment of VP2 encoding gene including GH loop region (267-498) of CPV-2 North Iraq isolate and a vaccine strain compared to the FJ222822.1_Duramune_DAPPI+LC strain. Samples of strain 2a, 2b, and 2c strains are also retrieved from the GenBank. The field sequence (KX198139- KX198141 and OL546608- OL546621).
Figure 3
Geographical distribution of CPV-2 variants in three regions of Iraq. new CPv-2a in Erbil and Kirkuk, new CPV-2b and CPV-2c in Sulaimani.
Figure 4
Phylogenetic relationships based on the complete VP2 gene of CPV-2 between field strains (north Iraq) isolates and reference strains. The analysis was performed employing the Neighbor-Joining method Based on 1000 replicates using MEGA X software. CPV field virus variants are indicated by and for new CPV-2a, new CPV-2b and CPV-2c, and commercial vaccine strain respectively.
June 2022
Vol. 10, Iss. 6, Pages 1189-1422
