Expression and Polymorphism Analysis of CFL2 Gene in Chinese Dabieshan Cattle
Expression and Polymorphism Analysis of CFL2 Gene in Chinese Dabieshan Cattle
Shuanping Zhao, Lei Xu, Hai Jin and Yutang Jia*
Deduced amino acid sequence of bovine CFL2 compared with human CFL2 (NP_001230574) and mouse CFL2 (NP_001830.1). Shading shows identical amino acid residues among the three species. Common structural domains are indicated by boxes including ADF domain.
Electrophoretic patterns of SNP genotyping by PCR-RFLP method; (a) is the PCR-HinfI-RFLP analysis result of SNP at g.1500G>A in intron 2 of the CFL2 gene, genotype GA (389 + 279 + 110 bp), genotype AA (279 + 110 bp) and genotype GG (389 bp); (b) is the PCR-VspI-RFLP analysis result of SNP at g.1694T>A in exon 4 of the CFL2 gene, genotype AT (197 + 172 + 25 bp, 25 bp fragment was too small to stay in gel), genotype TT (172 + 25 bp) and genotype AA (197 bp); (c) is the PCR- HaeIII-RFLP analysis result of SNP at g.2213C>G in 3’UTR of the CFL2 gene, genotype CG (276 + 252 + 24 bp; 24 bp fragment was too small to stay in gel), genotype CC (252 + 24 bp) and genotype GG (276 bp); M refers to the DNA molecular weight marker.
Expression pattern analysis of CFL2 mRNA in DBS cattle by real-time PCR method. The value of CLF2 in spleen was arbitrarily set to 1.0. The different letters (a, b, c and d) indicate a p-value of less than 0.05 in a Student’s t-test.
