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Evaluation of In Vitro Antibacterial Potential of Bacillus pseudomycoides Strain SB138

Evaluation of In Vitro Antibacterial Potential of Bacillus pseudomycoides Strain SB138

Rabiea Pervaiz, Shaista Bano*, Sarfraz Ali Tunio, Abdul Nabi Jatt and Aisha Amber Soomro

Institute of Microbiology, Allama I.I. Qazi Campus, University of Sindh, Jamshoro, Pakistan

 
* Corresponding author: shaista.bano@usindh.edu.pk

Fig. 1.

16S rRNA gene-based tree showing the phylogenetic relationship of the isolate SB 138 with type strain of B. pseudomycoides.

Fig. 2.

Colony characteristics of B. pseudomycoides SB138 a) the wild type B. pseudomycoides SB 138, and b) its colony mutant B. pseudomycoides SB138m.

Fig. 3.
Confirmation of the identification of B. pseudomycoides SB138m using 16S rRNA gene sequencing.
Fig. 4.

In vitro antibacterial activity of B. pseudomycoides strain SB138 against (a) indicator strain, S. aureus ATCC 6538 and (b) P. mirabilis 4S determined by stab- overlay methods.

Fig. 5.

Effect of (a) heat and (b) UV on antibacterial activity of B. pseudomycoides strain SB138m determined by spot-overlay method.

Fig. 6.

Determination of the effects of Proteinase K on antibacterial activity of B. pseudomycoides strain SB138m.

Pakistan Journal of Zoology

October

Pakistan J. Zool., Vol. 56, Iss. 5, pp. 2001-2500

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