Research Article

Epidemiological Survey of Fecal Parasites in Domesticated Doves in District Jhang, Pakistan

Muhammad Fiaz Qamar1*, Tahir Hussain1, Iram Liaqat2, Madiha Kiran1 and Abdur Rahman Ansari1

1College of Veterinary and Animal Sciences (CVAS), 12-Km, Chiniot Road, Jhang, Pakistan; 2Zoology Department, Government College University, Lahore, Pakistan.

Received | April 10, 2023; Accepted | June 07, 2023; Published | June 28, 2023

*Correspondence | Muhammad Fiaz Qamar, College of Veterinary and Animal Sciences (CVAS), 12-Km, Chiniot Road, Jhang, Pakistan; Email: [email protected]

Citation | Qamar, M.F., T. Hussain, I. Liaqat, M. Kiran and A.R. Ansari.2023. Epidemiological survey of fecal parasites in domesticated doves in district Jhang, Pakistan. Biologia (Lahore), 69(1): 15-19.

DOI | https://dx.doi.org/10.17582/journal.Biologia/2023/69.1.15.19

Keywords | Capillaria spp., Dove, Eimeria spp., Fecal examination

Copyright: 2023 by the authors. Licensee ResearchersLinks Ltd, England, UK.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

Introduction

Doves and pigeons representing the Columbidae family are found all over the world (Yabsley et al., 2015). These birds are used for the meat production and recreational purposes. Different parasites live in the gastrointestinal tract of the wild birds affecting the normal health status of the host, however most of the infections are subclinical but in some cases mortalities occur in the immuno-compromised birds (Lyles and Dobson, 1993; Atkinson et al., 2009). Parasites become pathogenic in the captive birds with high stocking densities. Effective management, health care and nutrition are necessary for the welfare of zoo species (Mellor et al., 2015). Fecal examination is the most frequently used method to determine the prevalence of endoparasites (Sood et al., 2018; Sobotyk et al., 2021).

Coccidia oocysts are usually found in the feces of captive and wild birds as Pathogenic Eimeria species causing the enteric diseases in the birds leading to huge economic losses, while diarrhea is caused by coccidiosis in many species of birds (Brice and Thomas, 2018). Columbiformes are occasionally described to have a high degree of diverse parasitism together with coccidia (Ortúzar-Ferreira et al., 2019). Coccidia is typically identified on the specific morphology along with quantification of oocysts and genotyping of particular genetic factors (Duszynski and Wilber, 1997; Tenter et al., 2002; Berto et al., 2011). Hence, the current study was conducted to find the incidence of fecal parasites in domesticated doves of Jhang district using the routine diagnostic methods.

Materials and Methods

Collection and examination of fecal samples

Fresh 400 fecal samples were collected from the local home-based fanciers and local markets of district Jhang in the plastic bags. All the samples were transported and processed through direct smear examination, sedimentation and flotation techniques for the detection of parasites in the Parasitology Laboratory at CVAS, Jhang. Briefly, 0.5 gram of fecal sample was mixed with 5 ml of distilled water, homogenized well and filtered through a sieve. Two drops were taken from the filtrate and placed on a glass slide and covered with a coverslip that was examined under the microscope (Nikon, Model Eclipse E200LED; Nikon Corporation, Tokyo Japan) for the detection of oocysts (Madlala et al., 2021). The positive samples were further determined through sedimentation and flotation methods.

The sedimentation method was performed as described by (Latif et al., 2016). In this procedure, 3 grams of the fecal sample was mixed with distilled water and sieved. Following that centrifugation was performed at 1500 rpm for two minutes. Subsequently, the supernatant was thrown away and a droplet of sediment material was placed on fresh and clean glass slide to detect the oocytes under microscope at 4X, 10X and 40X. The flotation technique was performed as described by (Faust et al., 1938). McMaster counting method was used for the counting of eggs per gram (EPG) in faecal sample as described by (Foreyt, 2013).

Statistical analysis

Statistical analyses were carried out by means of Quantitative Parasitology (QPweb) software Version 1.0.10 (Ham-Dueñas et al., 2017). Different parasitological parameters such as percentage prevalence, mean intensity and mean abundance were calculated following the standard statistical procedures characterized by (Rózsa et al., 2000).

Results and Discussion

Overall, 320 samples were found positive for one protozoan (Eimeria spp.) and one Helminth (Capillaria spp.) out of total sample examined. The doves were more frequently parasitized with the Eimeria spp. as compared to Capillaria species. The prevalence of Eimeria and Capillaria spp. were recorded as 75% and 25%, respectively in district Jhang. While, mixed infection (Eimeria spp. + Capillaria spp.) was not detected in any bird (Table 1). EPG counts for Coccidia and Capillaria were 700 and 850, respectively.

*No. of birds (infected)/total no. of birds examined (infected and non-infected) × 100

**Total no. of parasites within infected host/total no. of infected birds with that parasite

***Total no. of individual parasites in infected host/total no. of hosts examined (infected and non-infected)

The poultry birds that are kept in controlled sheds receive proper management, care, feeding, and vaccination against various harmful infections (Corkery et al., 2013). Due to proper hygienic measurements, they are less prone to parasitic disease (Kaboudi et al., 2016). However, doves are reared in the open houses and have unhygienic environment, mismanagement and neglected feeding without proper vaccination. Therefore, these birds are more prone to parasitic infection.

The prevalence of Eimeria and Capillaria spp. were recorded as 75% and 25%, respectively in district Jhang. There are different gastrointestinal parasites

 

Table 1: Measures of fecal parasites (prevalence, mean intensity and mean abundance) identified in dove fecal samples.

Parasite species (Dove)	Total infected birds (n)	Infected bird (n)	Not infected bird (n)	Prevalence infected birds (% )	Mean intensity	Mean abundance
Eimeria spp.	320	240	80	75	1.33	0.6
Capillaria spp.		80		25	4	0.2

 

that have been reported by various frequencies varying from 15 to 80 % around the globe (Faust and Pappas, 1977; Fallacara et al., 2004; Holsback et al., 2013; Hoque et al., 2014; Parsani et al., 2014). Eggs, larvae and oocysts have the main role for the transmission of various parasites from free range birds to zoo birds in the captivity through feces or ingestion of infected intermediate host (Schmidt and Roberts, 1989; Atkinson et al., 2009). Several scientific reports showed abundance of gastrointestinal parasites in wild birds (Fanelli et al., 2021), while the transmission of endo-parasites amongst avifauna in zoological localities in nearby zones is poorly studied yet. Carrera-Játiva et al. (2018) identified the several parasites of captive birds whereas the current study found the similar parasites of free-ranging birds that includes capillaria and Eimeria as the most abundant species (Carrera-Játiva et al., 2018). Coccidiosis was also reported in other poultry birds which usually limits the production and marketing of different meat-type birds especially pheasants, quails and chukar partridges (Mbong Ngwese et al., 2020; Madlala et al., 2021). In a prior study, Partridges showed no noticeable infection however, grey quails exhibited mild infection (Teixeira et al., 2015). In another study performed on two adult pigeons at Ludhiana, India, with the history of weakness, anorexia, ruffled feathers and intermittent diarrhea showed the coccidian oocysts alongside eggs of Capillaria spp. (Sood et al., 2018). Eimeria spp. was identified through fecal samples of doves (Zenaidamacroura) from Kentucky, USA (Yabsley et al., 2015) that resembles our current findings. Coccidia species have also been identified in columbiformes since long ago. Moreover, Eimeria spp. and Isospora spp. have been reported in columbiformes and identified on the basis of oocyst morphology (Ortúzar-Ferreira et al., 2019).

Eimeria was detected in 7% of 254 mourning doves that were inspected by direct fecal smear examination along with thickened duodenal mucosal lesions in some doves in several states of USA (Barrows and Hayes, 1977). Furthermore, 33% of 45 doves in several areas of northern Florida while 49% of 53 doves in the different zones of southern Florida were found infected with nonspecific type of Eimeria species (Conti and Forrester, 1981). It seems that the greater prevalence noticed in doves from Florida was probably linked to the usage of fecal flotation technique as compared to the direct smear method. In the present study, egg per gram (EPG) was recorded as moderate level of infection (700 for Eimeria spp. and 850 for Capillaria spp.) because of moderate temperature and moderate humidity in November, that are not very favorable conditions for parasitic infections. Similar findings were also reported by (Qamar et al., 2017).

Another scientific study described temperature and humidity with relation to level of infection as found in the current investigation (Parsani et al., 2014). They reported that nematode infection was found at moderate level when the temperature was low, high level of infection was attributed to monsoon weather (June to September) and the decreased rates were recorded during summer season (February to May). Moreover, the prevalence and level of parasitic infection is influenced by the epidemiological factors such as sex, age and breed (Djelmoudi et al., 2014).

Conclusions and Recommendations

Therefore, it is concluded that the doves were infected with different types of gastrointestinal parasites. This was the preliminary report on the prevalence of endoparasites in doves. Nevertheless, the prevalence of these infections in doves would be better confirmed by molecular techniques. Necessary prevention and control programs should be implemented to reduce the risk of doves to get parasitic infections. Furthermore, proper management tools and public awareness strategies should be applied to stop the spread of pathogens from doves to other livestock.

Author’s Contribution

MFQ: Supervised the entire work and wrote manuscript.

ARA and TH: Helped in planning of the experiment.

IL and ARA: Helped in the analysis and refining the manuscript.

IL: Helped in statistical analysis of data.

TH: Helped in collection of data.

Conflict of interest

The authors have declared no conflict of interest.

References

Atkinson, C.T., N.J. Thomas and D.B. Hunter, 2009. Parasitic diseases of wild birds. John Wiley & Sons. https://doi.org/10.1002/9780813804620

Barrows, P.L. and F.A. Hayes, 1977. Studies on endoparasites of the mourning dove (zenaida macroura) in the Southeast United States. J. Wildl. Dis., 13(1): 24-28. https://doi.org/10.7589/0090-3558-13.1.24

Berto, B.P., W. Flausino, D. McIntosh, W.L. Teixeira-Filho and C.W. Lopes, 2011. Coccidia of new world passerine birds (Aves: Passeriformes): A review of eimeria schneider, 1875 and isospora schneider, 1881 (Apicomplexa: Eimeriidae). Syst. Parasitol., 80(3): 159. https://doi.org/10.1007/s11230-011-9317-8

Brice, B. and G. Thomas, 2018. Coccidian tales. In: Proceedings of the Australian Wildlife Rehabilitation Conference.

Carrera-Játiva, P.D., E.R. Morgan, M. Barrows and T. Wronski, 2018. Gastrointestinal parasites in captive and free-ranging birds and potential cross-transmission in a zoo environment. J. Zoo Wildl. Med., 49(1): 116-128. https://doi.org/10.1638/2016-0279R1.1

Conti, J.A. and D.J. Forrester, 1981. Interrelationships of parasites of white-winged doves and mourning doves in florida. J. Wildl. Dis., 17(4): 529-536. https://doi.org/10.7589/0090-3558-17.4.529

Corkery, G., S. Ward, C. Kenny and P. Hemmingway, 2013. Incorporating smart sensing technologies into the poultry industry. J. World’s Poult. Res., 3(4): 106-128.

Djelmoudi, Y., M. Daoudi-Hacini and S. Doumandji. 2014. Common endoparasites of wild rock pigeon (Columba livia livia) and wood pigeon (Columba palumbus) in the algiers sahel, algeria. Int. J. Zoo, 4(3): 99-106.

Duszynski, D.W. and P.G. Wilber, 1997. A guideline for the preparation of species descriptions in the eimeriidae. J. Parasitol., pp. 333-336. https://doi.org/10.2307/3284470

Fallacara, D.M., C.M. Monahan, T.Y. Morishita, C.A. Bremer and R.F. Wack, 2004. Survey of parasites and bacterial pathogens from free-living waterfowl in zoological settings. Avian Dis., 48(4): 759-767. https://doi.org/10.1637/7185-032904R

Fanelli, A., P. Tizzani and E. Belleau, 2021. Gastrointestinal parasitic infection in the hazel grouse tetrastes bonasia in france. Wildl. Biol., 2021(2): wlb. 00731. https://doi.org/10.2981/wlb.00731

Faust, B.S. and P.W. Pappas, 1977. A survey of coccidia and helminth parasites of birds at the columbus (ohio) zoo. J. Zoo Anim. Med., 8(1): 18-23.

Faust, E.C., J.S. D’Antoni, V. Odom, M.J. Miller, C. Peres, W. Sawitz, L.F. Thomen, J. Tobie and J.H. Walker, 1938. A critical study of clinical laboratory technics for the diagnosis of protozoan cysts and helminth eggs in feces1. Am. J. Trop. Med. Hyg., 1(2): 169-183. https://doi.org/10.4269/ajtmh.1938.s1-18.169

Foreyt, W.J., 2013. Veterinary parasitology reference manual. John Wiley & Sons.

Ham-Dueñas, J., L. Chapa-Vargas, C. Stracey and E. Huber-Sannwald, 2017. Haemosporidian prevalence and parasitaemia in the black-throated sparrow (Amphispiza bilineata) in central-mexican dryland habitats. Parasitol. Res., 116(9): 2527-2537. https://doi.org/10.1007/s00436-017-5562-3

Holsback, L., M.J.L. Cardoso, R. Fagnani and T.H.C. Patelli, 2013. Natural infection by endoparasites among free-living wild animals. Rev. Brasil. Parasitol. Vet., 22(2): 302-306. https://doi.org/10.1590/S1984-29612013005000018

Hoque, M.A., M.M. Hassan, E. Haque, A.H. Shaikat, S.A. Khan, A. Alim, L.F. Skerratt, A. Islam, H.M. Tun and R. Dissanayake, 2014. A survey of gastro-intestinal parasitic infection in domestic and wild birds in chittagong and greater sylhet, bangladesh. Prevent. Vet. Med., 117(1): 305-312. https://doi.org/10.1016/j.prevetmed.2014.07.012

Kaboudi, K., S. Umar and M.T. Munir. 2016. Prevalence of coccidiosis in free-range chicken in sidi thabet, tunisia. Scientifica, 2016. https://doi.org/10.1155/2016/7075195

Latif, A.A., S. Fazal, F. Manzoor, A. Maqbool, S. Asghar, I. Wajid and A. Ashraf, 2016. A comparative study on prevalence of coccidian parasites in broiler chicken (Gallus gallus domesticus), Japanese quail (Coturnix coturnix japonica) and wild pigeon (Columba livia). Pak. J. Zool., 48(1): 295-297.

Lyles, A.M. and A.P. Dobson, 1993. Infectious disease and intensive management: Population dynamics, threatened hosts, and their parasites. J. Zoo Wildl. Med., pp. 315-326.

Madlala, T., M. Okpeku and M.A. Adeleke, 2021. Understanding the interactions between eimeria infection and gut microbiota, towards the control of chicken coccidiosis: A review. Parasite, 28. https://doi.org/10.1051/parasite/2021047

Mbong, N.M., G. Prince Manouana, P.A. Nguema Moure, M. Ramharter, M. Esen and A.A. Adégnika, 2020. Diagnostic techniques of soil-transmitted helminths: Impact on control measures. Trop. Med. Infect. Dis., 5(2): 93. https://doi.org/10.3390/tropicalmed5020093

Mellor, D.J., S. Hunt and M. Gusset, 2015. Caring for wildlife: The world zoo and aquarium animal welfare strategy. WAZA Executive Office.

Ortúzar-Ferreira, C.N., M.S. Oliveira, J.L. Genovez-Oliveira, H.A. Franco, S. Thode-Filho, S.V. Cardozo, Á.A. Oliveira, V.M. Lima, I. Ferreira and B.P. Berto, 2019. Coccidia of columbiformes: A taxonomic review of its Eimeriidae species and Eimeria columbinae n. Sp. From columbina talpacoti (temminck, 1809) from brazil. Parasitol. Res., pp. 1-15. https://doi.org/10.1007/s00436-019-06514-4

Parsani, H., R. Momin, A. Lateef and N. Shah, 2014. Gastro-intestinal helminths of pigeons (Columba livia) in gujarat, india. Egypt. J. Biol., 16(1): 63-71. https://doi.org/10.4314/ejb.v16i1.9

Qamar, M., A. Butt, S. Ehtisham-ul-Haque and M. Zaman. 2017. Attributable risk of capillaria species in domestic pigeons (Columba livia domestica). Arquivo Brasil. Med. Vet. Zoot., 69(5): 1172-1180. https://doi.org/10.1590/1678-4162-7829

Rózsa, L., J. Reiczigel and G. Majoros. 2000. Quantifying parasites in samples of hosts. J. Parasitol., 86(2): 228-233. https://doi.org/10.2307/3284760

Schmidt, G. and L. Roberts. 1989. Foundations of parasitology. Times mirror. Mosby College Publishing, St. Louis, Missouri.

Sobotyk, C., K.E. Upton, M. Lejeune, T.J. Nolan, A.E. Marsh, B.H. Herrin, M.M. Borst, J. Piccione, A.M. Zajac, L.E. Camp, C.N. Pulaski, L.A. Starkey, C. von Simson and G.G. Verocai, 2021. Retrospective study of canine endoparasites diagnosed by fecal flotation methods analyzed across veterinary parasitology diagnostic laboratories, united states, 2018. Parasites Vectors, 14(1): 439. https://doi.org/10.1186/s13071-021-04960-7

Sood, N., H. Singh, S. Kaur, A. Kumar and R.S. Singh, 2018. A note on mixed coccidian and capillaria infection in pigeons. J. Parasit. Dis., 42(1): 39-42. https://doi.org/10.1007/s12639-017-0961-z

Teixeira, M., A.D.B. Melo, G.R. Albuquerque, P.T. Rocha and J.P. Monteiro. 2015. Coccidia of gallinaceous meat birds in brazil. Rev. Brasil. Parasitol. Vet., 24(2): 230-234. https://doi.org/10.1590/S1984-29612015029

Tenter, A.M., J.R. Barta, I. Beveridge, D.W. Duszynski, H. Mehlhorn, D.A. Morrison, R.A. Thompson and P.A. Conrad, 2002. The conceptual basis for a new classification of the coccidia. Int. J. Parasitol., 32(5): 595-616. https://doi.org/10.1016/S0020-7519(02)00021-8

Yabsley, M.J., K. Bailey and H.C. Adams, 2015. A new species of eimeria (apicomplexa: Eimeriidae) from the mourning dove, zenaida macroura (columbiformes: Columbidae). Comp. Parasitol., 82(2): 231-234. https://doi.org/10.1654/4769.1