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Effects of Green Tea Polyphenols, Vitamin E, and Ocimum gratissimum Leaf Essential Oils as a Supplement to Extender on Chilled Canine Sperm Quality

Effects of Green Tea Polyphenols, Vitamin E, and Ocimum gratissimum Leaf Essential Oils as a Supplement to Extender on Chilled Canine Sperm Quality

Vui Van Nguyen1, Samorn Ponchunchoovong2, Sajeera Kupittayanant3, Pakanit Kupittayanant2* 

1Department of Animal Science and Veterinary Medicine, Faculty of Agriculture and Aquaculture, Tra Vinh University, Vietnam; 2School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Thailand; 3School of Preclinical Sciences, Institute of Science, Suranaree University of Technology, Thailand.

*Correspondence | Pakanit Kupittayanant, School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Thailand; Email: [email protected] 

Figure 1

Canine sperm stained with the combination of H324, PI, FITC-PSA, and JC-1 under a confocal laser scanning microscope (600x magnification). (a) Sperm with high mitochondrial membrane potential, intact plasma and acrosome membrane. (b) Sperm with high mitochondrial membrane potential, intact plasma membrane, and damaged acrosome membrane, and. (c) Sperm with high mitochondrial membrane potential, damaged plasma membrane, and intact acrosome membrane. (d) Sperm with low mitochondrial membrane potential, intact plasma membrane, and damaged acrosome membrane. (e) Sperm with low mitochondrial membrane potential, damaged plasma membrane, and intact acrosome membrane. (f) Sperm with low mitochondrial membrane potential, damaged plasma and acrosome membrane. 

Advances in Animal and Veterinary Sciences

November

Vol. 12, Iss. 11, pp. 2062-2300

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