Effect of a Polymorphism in Prolactin Gene on Some Reproductive Traits in TB Crossbred Ducks in Southern Vietnam
Effect of a Polymorphism in Prolactin Gene on Some Reproductive Traits in TB Crossbred Ducks in Southern Vietnam
Ngoc Tan Nguyen1*, Tan Loi Le1, Thi Kim Ngan Vo1, Chi Hieu Do1, Tuan Thanh Hoang2, Nguyen Khang Duong3, Quang Minh Luu4
Representative duck mating schemes to produce TB crossbred duck (left) and parents and TB crossbred ducks (right).
Representative electrophoresis of the PCR products of prolactin in exon 5 on agarose gel. L: DNA ladder (100 bp). No. 1-11: individual samples; (-): Negative control.
Representative PCR-RFLP pattern of IGF-1 gene digested with PstI and separated on 2% agarose gel, L: DNA ladder (100 bp); lane 2, 5, 8, 10: CC genotype (536 bp); lane 1, 3, 4, 6, 11: CT genotype (536/406/130 bp); lane 9: TT (406/130 bp); (-) negative control (PCR product without enzyme treatment).
Partial sequencing of exon 5 prolactin gene showing mutation sites; A: CC genotype; B: CT genotype; C: TT genotype; 403: indication of mutation site based on sequences used.
