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Effect of Different Strengths of MS Media and BAP on Banana Plantlet Growth in Vitro

Effect of Different Strengths of MS Media and BAP on Banana Plantlet Growth in Vitro

Pangesti Nugrahani1*, Hery Purnobasuki2, Arif Nur Muhammad Ansori3, Jatuporn Anuchai4 and Anugerah Dany Priyanto5,6

1Department of Agrotechnology, Faculty of Agriculture, Universitas Pembangunan Nasional Veteran Jawa Timur, Surabaya, Indonesia; 2Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia; 3Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Dehradun, India; 4School of Industrial Education and Technology, King Mongkut’s Institute of Technology Ladkrabang, Ladkrabang, Thailand; 5Department of Food Technology, Faculty of Engineering, Universitas Pembangunan Nasional Veteran Jawa Timur, Surabaya, Indonesia; 6Innovation Center of Appropriate Food Technology for Lowland and Coastal Area, Universitas Pembangunan Nasional Veteran Jawa Timur, Surabaya, Indonesia.

 
*Correspondence | Pangesti Nugrahani, Department of Agrotechnology, Faculty of Agriculture, Universitas Pembangunan Nasional Veteran Jawa Timur, Surabaya 60294, Indonesia; Email: [email protected]

ABSTRACT

Banana tissue culture is commonly conducted using Murashiage and Skoog (MS) media supplemented with various types of growth regulators (PGRs). Cytokinin and Auxin are incorporated into MS media to promote shoot and root growth in banana explants. 6-benzyl amino purine (BAP), a form of cytokinin, plays a significant role in stimulating cell division and differentiation. This study aimed to assess the impact of BAP on various growth parameters, including shoot initiation, root initiation, leaf initiation, shoot and root length, plantlet weight, root number, leaf number, and shoot number. The study followed a completely randomized design with one factor, namely the composition of the planting medium, consisting of five treatments: full MS without BAP (MS0), ½ MS without BAP (½MS0), ¼ MS without BAP (¼ MS0), ½ MS supplemented with BAP at 3 mg L-1 (½ MSB), and ¼ MS supplemented with BAP at 3 mg L-1 (¼ MSB). The results revealed that the timing of shoot, root, and leaf initiation was not significantly altered by any of the treatments. However, shoot and root lengths were notably influenced by the treatment without BAP. Intriguingly, treatments without BAP consistently yielded superior growth outcomes, as evident from root growth and the number of leaves observed over 56 days after planting. The treatment of ¼ MS without BAP demonstrated the highest number of roots, with the ½ MS0 treatment displaying the highest number of leaves. In conclusion, utilizing MS growth media without BAP in banana tissue culture resulted in plantlets with robust growth and vigor, although the production of shoots was limited. The addition of 3 mgL-1 BAP at 1/2 MS gave the best number of shoots.

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Pakistan Journal of Zoology

December

Pakistan J. Zool., Vol. 56, Iss. 6, pp. 2501-3000

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