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Dromedary Camel (Camelus dromedarius) Cloning Using Somatic Cell Nuclear Transfer (SCNT) Current Knowledge and Future Perspectives

Dromedary Camel (Camelus dromedarius) Cloning Using Somatic Cell Nuclear Transfer (SCNT) Current Knowledge and Future Perspectives

Fahad A. Alshanbari1, Abdelrahman M. A. Elseory2,3* 

1Department of Medical Biosciences, College of Veterinary Medicine, Qassim University, Buraydah 51452, Saudi Arabia; 2Department of Anatomy, College of Veterinary Medicine, King Faisal University, Saudi Arabia, P.O. Box 400 Al-Ahsa 31982, Saudi Arabia; 3Department of Anatomy, Faculty of Veterinary Medicine, University of Khartoum. PO Box: 32, Postal Code: 13314 Shambat, Khartoum North, Sudan.

*Correspondence | Fahad A. Alshanbari, Department of Medical Biosciences, College of Veterinary Medicine, Qassim University, Buraydah 51452, Saudi Arabia; Email: [email protected] 

Figure 1

Different ages of dromedary camels representing the simple life cycle of the dromedary. A: 3 months old calf; B: 2.5 years old juvenile (immature male); C: 4.5 years old female (unmated female); D: 12 years old pregnant female (8 months pregnancy); E: 8 years old female with newborn calf and F: 18 years old fertile bull in reproduction season. 

Figure 2

A: A donor dromedary to be cloned; B: Skin tissue; C: Fibroblast created from skin tissue; D: A fibroblast cell picked from the cell culture; E: Dromedary female; F: Oocyte picked from the female; G: Microneedle used for enucleation; H: Insertion of the fibroblast into the oocyte; I: Insertion of the picked fibroblast into the oocyte; J: Electroshock to activate the cloned oocyte and fibroblast; K: Cloned zygote; L: 2 cells cleavage; M: 4 cells cleavage; N: 8 cells cleavage; O: Morula; P: Blastula; Q: Transfer blastula to the recipient; R: Cloned embryo that is identical to A. 

Pakistan Journal of Zoology

October

Pakistan J. Zool., Vol. 56, Iss. 5, pp. 2001-2500

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