Diagnosis of Staphylococcus aureus Infection in Bovine Mastitis using its Affinity Purified Fraction

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Diagnosis of Staphylococcus aureus Infection in Bovine Mastitis using its Affinity Purified Fraction

Ehab A. Fouad¹*, Khaled A. Abd El-Razik² , Eman H. Abdel-Rahman³

Author Affiliations

¹Microbiology and Immunology Department, National Research Centre, Giza, Egypt; ²Animal Reproduction Department, National Research Centre, Giza, Egypt; ³Parasitology and Animal Diseases Department, National Research Centre, Giza, Egypt

*Correspondence | Ehab Fouad, Microbiology and Immunology Department, National Research Centre, Giza, Egypt; Email: [email protected]

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Figure 1:

SDS PAGE profile of S. aureus purified fraction showed 2 bands (2 peaks).

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Figure 2:

SDS PAGE profile of S. aureus Crude Antigen show 10 bands (10 peaks).

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Figure 3:

Mean OD values of cows’ positive sera against the crude antigen of S. aureus.

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Figure 4:

Mean OD values of cows’ positive sera against the purified fraction of S. aureus.

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Figure 5:

Potency of the purified fraction of S. aureus to antibodies of positive cows’ milk samples.

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Figure 6:

Significant validity of the stored purified fraction of S. aureus for one year at -20oC.

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Figure 7:

Validity evaluation of the stored purified (Series 2) S. aureus fraction for one year at -20oC compared with freshly purified fraction (Series 1).

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Figure 8:

Specificity evaluation of S. aureus purified fraction in comparison with different antigens in diagnosis of mastitis.

This Issue

September 2022

Vol. 10, Iss. 9, Pages 1887-2089

Advances in Animal and Veterinary Sciences

Diagnosis of Staphylococcus aureus Infection in Bovine Mastitis using its Affinity Purified Fraction

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Diagnosis of Staphylococcus aureus Infection in Bovine Mastitis using its Affinity Purified Fraction

Ehab A. Fouad¹*, Khaled A. Abd El-Razik² , Eman H. Abdel-Rahman³

Advances in Animal and Veterinary Sciences

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Advances in Animal and Veterinary Sciences

Diagnosis of Staphylococcus aureus Infection in Bovine Mastitis using its Affinity Purified Fraction

Special Issues

Membership/Association

Diagnosis of Staphylococcus aureus Infection in Bovine Mastitis using its Affinity Purified Fraction

Ehab A. Fouad1*, Khaled A. Abd El-Razik2 , Eman H. Abdel-Rahman3

Advances in Animal and Veterinary Sciences

December

Featuring

Study of Noni Fruit Extract Microcapsule on Enzymes Activity, Digestibility and Hematologic of Sentul Chicken

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Primary, Secondary Metabolites and Antioxidant Activity of Castanopsis Argantea, Artocarpus heterophyllus, and Aglaia tomentosa Fruit as Food for the Tapanuli Orangutan (Pongo tapanuliensis) in the Batang Toru Forest, North Sumatra

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Ehab A. Fouad¹*, Khaled A. Abd El-Razik² , Eman H. Abdel-Rahman³