Detection of Streptococcus thoraltensis in Raw Milk with Special Reference to Their Antibiogram
Detection of Streptococcus thoraltensis in Raw Milk with Special Reference to Their Antibiogram
Hams M.A. Mohamed1, Mona A. El-Zamkan2*
Proteolytic activity of S. thoraltensis on skim milk agar.
PCR products of the antimicrobial resistance genes (a, b, c and d) identified in S. thoraltensis visualized on agarose gel electrophoresis. The expected molecular size of amplified DNA: 430 bp for Pbp1A gene (a), 732 bp for vanA gene (b), 425bp for ermB gene (c) and 1395 bp for optrA gene (d). Lane 1-4: samples, Lane (L): DNA ladder, Lane (+ve): positive control and Lane (-ve): negative control.
PCR products of the genes encoding for biofilm production (a and b) identified in S. thoraltensis visualized on agarose gel electrophoresis. The expected molecular size of amplified DNA: 534 bp for brpA gene (a) and 397 bp for lmb gene (b). Lane 1 and 2: samples, Lane (L): DNA ladder, Lane (+ve): positive control and Lane (-ve): negative control.
Heat map summary of phenotypic and genotypic characters of S. thoraltensis isolates.