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Detection and Genotyping of Toxoplasma gondii in Beef and Mutton Consumed in Gaza, Palestine

Detection and Genotyping of Toxoplasma gondii in Beef and Mutton Consumed in Gaza, Palestine

Zuhair Dardona1,2, Adnan Al-Hindi3, Mohamed Hafidi1, Ali Boumezzough1 and Samia Boussaa1,4*

1Microbial Biotechnologies, Agrosciences and Environment (Labelled Research Unit N°4, CNRST), Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakesh, Morocco.
2Governmental Medical Services, Gaza, Palestine.
3Faculty of Health Sciences, Islamic University of Gaza, Gaza, Palestine. P. O. Box 108, Gaza Strip, Palestine.
4ISPITS-Higher Institute of Nursing and Health Techniques, Ministry of Health and Social Protection, Rabat, Morocco.
 
* Corresponding author: samiaboussaa@gmail.com

Fig. 1.

Results of T. gondii recovery test of red meat samples (Beef and Mutton). B1 amplification products loaded on to 2% gel agarose. Lane M, molecular weight marker (Marker 50 bp, Bioline, Italy). Lane N, Negative control. Lane P positive control. Lanes 3 and 5 positive samples, and Lanes 1, 2, and 4, negative samples.

Fig. 2.

PCR-RFLP pattern of the GRA6 gene cut by MseI endonuclease (75 and 623bp bands), corresponding to the pattern of type II T. gondii on a 2% agarose gel.

Pakistan Journal of Zoology

June

Pakistan J. Zool., Vol. 56, Iss. 3, pp. 1001-1500

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