Comparative Evaluation of Elisa Using A Semi-Nested 16SrRNA Pcr as a Master Test for Detection of Helicobacter Pylori Antigen in Human Stool and Feces of Dogs and Cats
Comparative Evaluation of Elisa Using A Semi-Nested 16SrRNA Pcr as a Master Test for Detection of Helicobacter Pylori Antigen in Human Stool and Feces of Dogs and Cats
Neven Waheeb1*, Sherif Marouf2, Essam Nasr3, Shaymaa Abdelmalek2
Agarose gel electrophoresis (1%) of 16sRNA using nested PCR showing; first run of heminested PCR using outer primers showing 1024 bp product and second run PCR using heminested primers. 250 bp product.