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Comparative Evaluation of Elisa Using A Semi-Nested 16SrRNA Pcr as a Master Test for Detection of Helicobacter Pylori Antigen in Human Stool and Feces of Dogs and Cats

Comparative Evaluation of Elisa Using A Semi-Nested 16SrRNA Pcr as a Master Test for Detection of Helicobacter Pylori Antigen in Human Stool and Feces of Dogs and Cats

Neven Waheeb1*, Sherif Marouf2, Essam Nasr3, Shaymaa Abdelmalek2 

1Veterinary Serum & Vaccine Research Institute - VSVRI, Egypt; 2Microbiology and immunology Faculty of Veterinary Medicine, Cairo University, Egypt; 3Head of Bacteriology Diagnostic Products Department Tuberculosis)Veterinary Serum and Vaccine Research Institute Abbasia, Cairo **Vice Chairman of the Vaccine Research Institute Abbasia, Cairo, Egypt.

*Correspondence | Neven Waheeb, Veterinary Serum & Vaccine Research Institute - VSVRI, Egypt; Email: [email protected] 

Figure 1

Agarose gel electrophoresis (1%) of 16sRNA using nested PCR showing; first run of heminested PCR using outer primers showing 1024 bp product and second run PCR using heminested primers. 250 bp product. 

Advances in Animal and Veterinary Sciences

December

Vol. 12, Iss. 12, pp. 2301-2563

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