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Cloning and Expression of Interleukin 11 in Bacillus subtilis

Cloning and Expression of Interleukin 11 in Bacillus subtilis

Atena Azarniush1, Majid Moghbeli2*, Farshid Kafilzadeh1, Mohammad Kargar1 and Hooshang Jamali1

1Department of Microbiology, Islamic Azad University, Jahrom Branch, Jahrom, Iran
2Department of Biology, Islamic Azad University, Damghan Branch, Damghan, Iran
* Corresponding author:

Fig. 1.

The sequence of the designed and synthesized interleukin 11gene based on the codon usage in B. subtilis: The underlined sequences are restriction sites: 5′ end and 3′ end respectively belongs to BamHI and XbaI.

Fig. 2.

A, Double digestion of the plasmid pHT43 containing IL-11 gene: lane 1: 1 kb plus DNA ladder; lane 2: double digested recombinant plasmid. B, PCR of the recombinant plasmid pHT-MA52: lane 1: negative control. Lane 2: PCR product. Lane 3: 1 kb plus DNA ladder.

Fig. 3.

Evaluation of the recombinant protein band on SDS-PAGE: lane 1: negative control, lane 2: recombinant protein, lane 3: protein ladder. 

Pakistan Journal of Zoology


Vol. 55, Iss. 3, Pages 1003-1500


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