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Characterization of β-casein Gene Sequence Variants in Cholistani Cattle

Characterization of β-casein Gene Sequence Variants in Cholistani Cattle

Ruqayya Bint Khalid1, Asif Nadeem1,2*, Maryam Javed1, Muhammad Zubair Shabbir3 and Masroor Ellahi Babar4

1Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan. 
2Department of Biotechnology, Virtual University of Pakistan, Lahore, Pakistan
3Department of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan 
4The University of Agriculture, Dera Ismail Khan, Khyber Pakhtunkhwa, Pakistan

*      Corresponding author: asif.nadeem@vu.edu.pk

Fig. 1.

At position 85451290 of β-casein gene, AGG codon presence confirms the presence of A2 allele in cholistani cattle of Pakistan.

Fig. 2.

Multiple sequence alignment of nine β-casein protein sequences of different species.

Fig. 2.

Multiple sequence alignment of nine β-casein protein sequences of different species.

Fig. 3.

Phylogenetic tree of β-casein gene of nine species constructed with the help of Neighbor-Joining method with bootstrap of 1000 iterations and Maximum composite likelihood method.

Fig. 4.

Protein structure of wild β-casein protein (a), mutant β-casein protein (b).

Pakistan Journal of Zoology

October

Vol. 53, Iss. 5, Pages 1603-2000

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