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Cellulase Production by Trichoderma viride in Submerged Fermentation using Response Surface Methodology

Cellulase Production by Trichoderma viride in Submerged Fermentation using Response Surface Methodology

Tehreem Zahra1, Muhammad Irfan1, Muhammad Nadeem2, Misbah Ghazanfar1, Qurratulain Ahmad3, Shaukat Ali4, Farzana Siddique5, Zarina Yasmeen6, Marcelo Franco7

1Department of Biotechnology, University of Sargodha, Sargodha, Pakistan
2Food and Biotechnology Research Center, PCSIR Labs. Complex, Ferozpure Road, Lahore, Pakistan
3Institute of Biochemistry and Biotechnology, University of the Punjab, New Campus, Lahore. 
4Department of Zoology, Government College University, Lahore, Pakistan
5Institute of food science and nutrition, University of Sargodha, Sargodha, Pakistan
6Post Harvest Research Centre, Ayub Agriculture Research Institute, Faisalabad, Pakistan
7Department of Exact Sciences and Technology, State University of Santa Cruz (UESC), Ilhéus, 45662-900 Brazil

Corresponding Author: Muhammad Irfan
irfan.ashraf@uos.edu.pk, irfan.biotechnologist@gmail.com

Figure 1:

Predicted vs observed values for CMCase and FPase.

Figure 2:

Contour plots for CMCase (A) and FPase (B) production from Trichoderma viride under SmF.

Figure 3:

Desirability chart for CMCase production. Var1; substrate concentration, var2; Peptone, var3; KH2PO4.

Figure 4:

Desirability chart for FPase production. Var1; substrate concentration, var2; Peptone, var3; KH2PO4.

Punjab University Journal of Zoology

June

Vol.38, Iss. 1, Pages 01-135

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