Cardioprotective Effect of Spinosin against Doxorubicin Induced Inflammatory and Hypertrophy via Nrf2 Signaling
Cardioprotective Effect of Spinosin against Doxorubicin Induced Inflammatory and Hypertrophy via Nrf2 Signaling
Mohammad Bani Ismail
SPN inhibits cell death in Dox induced cardiac cells. (A and B) Cell growth effect of SPN indicated time and dose by MTT. (C) Dox cell toxicity assay by MTT. (D) Cell growth effect of SPN on Dox induced H9c2 cells. P<0.05 significantly different from the control group.
SPN inhibits the cardiac abnormalities in Dox induced cardiac cells. (A) Cardiac abnormalities marker ANP and BNP expression analyzed by western blot. (B) Effect of SPN on calcinurin and phosphorylation of GATA4 in Dox induced H9c2 cells. P<0.05 significantly different from the control group.
SPN on pro-inflammatory cytokine expression Dox induced cardiac cells. (A) Epression of pP65, IL-6 TNF-α and COX-2 by western blot. (B) Actin filament changes control and treatment groups. P<0.05 significantly different from the control group.
Effect of SPN on Dox induced apoptosis. (A) Cell survival protein Bcl2 and BclxL and apoptotic marker caspase-3 analyzed by WB. (B) Apoptosis was analyzed by TUNEL assay. P<0.05 significantly different from the control group.
SPN on Nrf2 signaling. (A) WB showing expression of total Nrf2, HO-1 and NQO-1 control and treatment groups. (B) Effect of SPN on Nrf2 nuclear translocation. (C) ROS formation. P<0.05 significantly different from the control group.
