Analysis of Mitochondrial COI Gene for Species/Breed Level Identification of the Genus Capra (Linnaeus, 1758)
Analysis of Mitochondrial COI Gene for Species/Breed Level Identification of the Genus Capra (Linnaeus, 1758)
Bibi Malaela1*, Nabeela Tariq1, Nazeer Ahmed2, Hina Ali Ahmed1, Asmatullah Kakar3*, Sidra Khan Kasi1, Nida Ali2 and Nisar Ahmed2
Multiple sequence alignment of goat breeds represented one SNP.
Total two SNPs were found in the intraspecific Multiple sequence alignment of goat breeds.
Evolutionary analysis was conducted on MEGAX. The evolutionary history was inferred using the Neighbor joining tree method and the evolutionary distances were computed by using the p-distance method (d). The percentage of replicating trees in which the associated taxa clustered together in the bootstrap test of 1000 replicates. There were a total of 528 positions in the final dataset.
Neighbor joining tree was constructed between the wild species and domestic goats and confirmed the lowest genetic distance (0.01%) as the Capra sibirica appeared in the same cluster with the domestic breeds of goat.
The evolutionary history was inferred using the neighbor-joining method. The percentage of replicating trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) is shown next to the branches. The evolutionary distances were computed using the p-distance method (d). The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. There were a total of 1542 positions in the final dataset.
