Ameliorating Effect of Linseed Oil Against Fluconazole Induced Adverse Effect on Male Fertility of Cocks
Ameliorating Effect of Linseed Oil Against Fluconazole Induced Adverse Effect on Male Fertility of Cocks
Nour El-Hoda Khayrat Hammad1*, Yousef Y. El-Seady3, Azza E. Hassan1, Sara T. Elazab2, Magdy S. Amer2
Showing sperm morphology of clinically healthy adult cocks. A and B: normal sperm (arrow) in control group G1, C: large head sperm (arrow) in G2, D: bend middle piece sperm (arrow) in G3 (X: 400-1000). G1: Control (non-treated); G2: Fluconazole (5mg/kg b.wt); G3: Fluconazole (5mg/kg b.wt) then Linseed oil (60ml/kg).
(A and B) Testis, Gp1 control showing compact seminiferous tubules with narrow lumen (double headed arrow) and lined with several layers consisting of spermatogonea cells (1), spermatocytes (2), Sertoli cell (3), spermatid (4) and spermatozoids (5). (C and D), Gp2 showing wider lumen (double headed arrow) containing large amount of exfoliated germ cells (black arrow) and vacuolated spermatocytes (arrowhead). (E and F), Gp3 showing wide lumen (double headed arrow) and mildly vacuolated spermatocytes (arrowhead). H and E, Low magnification X: A, C and E:100 bar 100 and high magnification X: B, D and F:400 bar 50.
(A and B) Epididymis, Gp1 control showing epididymal duct with great quantity of spermatozoids (asterisk) in lumen and normal epithelial lining (white arrow), (C, D and E), Gp2 showing marked decrease in diameter of epididymal duct and amount of spermatozoids in lumen with presence of large amount of exfoliated germ cells and round bodies in the lumen (black arrows), hyperplastic alteration in the lining epithelium forming infolding or cribriform change and pseudoglandular structures (blue arrows) besides hyperplasia of clear cells (red arrow). (F and G), Gp3 showing slight increase in amount of spermatozoids in lumen (asterisk) with slightly decreased hyperplastic alteration in the lining epithelium (blue arrows) besides presence of few exfoliated germ cells and round bodies in the lumen (black arrows). H and E, Low magnification X: A, C and F: 100 bar 100 and high magnification X: B, D, E and G:400 bar 50.
Effect of linseed oil (60ml/kg) and fluconazole (5mg/kg b.wt) in 3rd week post fluconazole administration on fertility related genes of clinically healthy adult cocks. G1: Control negative (non-treated); G2: Control positive (treated with Fluconazole 5mg/kg b.wt); G3: Fluconazole (5mg/kg b.wt) then Linseed oil (60ml/kg).
