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Short Communication · Volume 4, Issue 12, Pages 637–641, 1 January 2017 · Open Access

Isolation and Characterization of Klebsiella pneumoniae from Respiratory Infection of Yak (Bos grunniens), India

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Advances in Animal and Veterinary Sciences
Published:
1 January 2017
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From the article

  • Isolation and Characterization of Klebsiella pneumoniae from Respiratory Infection of Yak (Bos grunniens), India.
  • Nasal swab sample was collected from pneumonic yak in Amies charcoal transport media from Arunachal Pradesh, India and processed for bacterial isolation and identification and direct PCR detection in 18h BHI enriched broth sample.
  • The 16S rRNA sequence analysis and 16S rRNA secondary structure prediction revealed close geographical relatedness to environmental K. pneumoniae PB12 from River Mahananda from East India and K. pneumoniae JPR 9 isolated from soil samples of Assam.
  • This study describes pneumonia in yak due to K. pneumoniae from Arunachal Pradesh, India by direct PCR detection from enriched clinical sample, isolation, PCR and 16S rRNA sequence, phylogenetic and secondary structure relation studies.

Selected from the article's own text.

Abstract

Swati Sahay, Juwar Doley, Natesan Krithiga, Gundallahalli Bayyappa Manjunatha Reddy, Siddanna Sharangouda Patil, Bibek Ranjan Shome, Rajeswari Shome 

ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (ICAR-NIVEDI), Yelahanka, Bengaluru–560064, India; 2ICAR-National Research Centre on Yak, Dirang-790 101, Arunachal Pradesh, India.
 
Abstract | Yak (Bos grunniens) is multipurpose long haired ruminant reared by the poor tribal farmers for wool, milk, meat, etc in the high altitudes of Himalayan region of Indian Territory. There is a serious concern about increasing morbidity and mortality of yak due to respiratory diseases. Nasal swab sample was collected from pneumonic yak in Amies charcoal transport media from Arunachal Pradesh, India and processed for bacterial isolation and identification and direct PCR detection in 18h BHI enriched broth sample. Based on biochemical characteristics and multiplex PCR, the culture was identified as K. pneumoniae (isolate No. KP1) and in in-vitro antibiotic sensitivity test, the isolate was resistant to ampicillin. The 16S rRNA sequence analysis and 16S rRNA secondary structure prediction revealed close geographical relatedness to environmental K. pneumoniae PB12 from River Mahananda from East India and K. pneumoniae JPR 9 isolated from soil samples of Assam. This study describes pneumonia in yak due to K. pneumoniae from Arunachal Pradesh, India by direct PCR detection from enriched clinical sample, isolation, PCR and 16S rRNA sequence, phylogenetic and secondary structure relation studies.

Keywords | Isolation, Klebsiella pneumoniae, Pneumonia, 16S sequencing, Yak

 

Acknowledgments

We are thankful to the Director and scientists, ICAR-National Research Centre on Yak, Dirang, Arunachal Pradesh for their support & collaboration for sharing samples. This work is funded by Department of Biotechnology Govt. of India through twinning project entitled “Aetio-Pathology and molecular epidemiology of bacterial and viral diseases associated with the respiratory problems of yak in the North Eastern Regions of India (Grant No: BT/391/NE/TBP/2012)”.

Conflict Of Interest

None of the authors of this paper has a financial or personal relationship with other people or organizations that could inappropriately influence or bias the content of the paper.

Authors’ Contribution

RS designed the study and corrected the manuscript. JD provided the sample for study. SS, KN carried out the experimental work, analyzed the data and drafted the manuscript. GBMR,SSP, BRS provided guidance and support. All authors read and approved the final manuscript.

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