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Short Communication · Volume 54, Issue 1, 27 December 2021 · Open Access

Epidemiological and Clinical Features of Fasciola hepatica Infection in Yaks (Bos grunniens) on Tibetan Plateau, China

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Affiliations & Notes ⌄
  1. 1College of Animal Science, Tibet Agriculture and Animal Husbandry University Linzhi 860000 Tibet, China 2Animal Husbandry and Veterinary station, Bureau of Agriculture and Rural Affairs, Linzhi 860000 Tibet, China 3University College of Veterinary and Animal Sciences, the Islamia University of Bahawalpur, 63100, Pakistan 4Department of Veterinary, Tibet Livestock Research Institute, Tibet Academy of Agriculture and Animal Science, Lhasa, Tibet, China 5Department of Parasitology, University of Ag
Article Info ⌄
Journal:
Pakistan Journal of Zoology
Published:
27 December 2021
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From the article

  • Our results showed that the seroprevalence of F. hepatica in yaks was high in Gongbo’gyamda and Mainling.
  • The seroprevalence of F. hepatica increased from 20.75%-48.8% with age of yaks, with the further distribution of 20.75%, 30.23%, 39.55%, 37.70% and 48.8% for age groups: 1-2 year, >2-4 years and >4 years, respectively.
  • The seroprevalence in male and female yaks was 32.51% and 39.05%, respectively (Table I).
  • The gender based results have no statistically significant to influence the seroprevalence of F. hepatica among yaks, it has also suggested that the incidence of F. hepatica case have no relationship with exact gender.

Selected from the article's own text.

Abstract

Fascioliasis is a common foodborne and waterborne zoonotic trematode parasitic disease infection worldwide. However, yak’s fascioliasis is rarely reported on Tibetan Plateau. In this study, serological survey and associated risk factors of Fasciola hepatica (F. hepatica) infection in Tibetan yaks was investigated using an ELISA assay. The results showed that the 305/849 (35.92%) studied animals were sero-positive for F. hepatica with the further distribution of 24.20% (38/157), 38.02% (92/242) and 38.89% (175/450) from Bayi, Mainling, and Gongbo’gyamda areas, respectively. The seroprevalence of F. hepatica increased from 20.75% to 48.8% with age of yaks, with the further distribution of 20.75%, 30.23%, 39.55%, 37.70% and 48.8% for age groups: <0.5 year, 0.5-1 year, >1-2 year, >2-4 years and >4 years, respectively. The prevalence in the male and female yaks was 32.51% and 39.05%, respectively. The physical examination showed a poor body condition with emaciation, weakness, loss of appetite, anemia, jaundice, and abdominal edema in infected yaks, while on dissection, many prominent nodules of parasitic migration were present on the liver surface. Our results provided a baseline data for the exposure of yaks to F. hepatica infection in the study area. Therefore, some important strategies and surveillance should be adopted to control F. hepatica on Tibetan Plateau, China.

Materials and methods

The study was permitted by the ethics committee of Tibet Agriculture and Animal Husbandry University (Permit number: 2018040501). A total of 849 blood samples were collected from the jugular vein by local veterinary practitioners from three regions of Tibet (Bayi, Mainling, and Gongbo’gyamda) from 2018 to 2019 (Supplementary Fig. 1). The present study was carried out in Nyingchi, in southeastern Tibet, China. This area is geographically isolated from Tibet and Sichuan Provinces by Himalayas, and shares borders with Yunnan and Qinghai Provinces (Wu et al., 2018a; . The average height of the altitude is about 3100 meters in the study site (Li et al., 2019a, b). All collected samples were centrifuged at 3500 × g for 10-20 min, and serum was used for further analysis. A total of 10 yaks with typical symptoms of F. hepatica infection were slaughtered at local slaughterhouses. After autopsy, the prominent nodules of parasitic migration in the surface of livers were examined, and the severity of parasitic infection were recorded.

The serum samples were tested by using a commercial ELISA kits according to the manufacturer’s instructions (Shanghai Yuduo, Shanghai, China). Briefly, the critical value (cut off) was calculated as: cut off = Negative control + 0.15; if the samples OD450 < cut off were interpreted as negative, while the samples OD450 > cut off were considered as positive.

The data were analyzed with Chi-square test (SPSS, version 21.0). Furthermore, the odds ratios (OR) were also calculated based on likelihood ratio.

Results and discussion

F. hepatica infection results in significant losses because of mechanical injury of liver, hepatitis, cholangitis, and even death of animals. A total of 305 out of 849 serum samples (35.92%) were found positive to F. hepatica in yaks trough ELISA test, with the further distribution of 24.20% (38/157), 38.02% (92/242) and 38.89% (175/450) from Bayi, Mainling, and Gongbo’gyamda areas, respectively. Our results showed that the seroprevalence of F. hepatica in yaks was high in Gongbo’gyamda and Mainling. It may be related to climatic conditions of this area with a high temperature, humidity, rainfall, and other weather conditions which are favorable for the existence and spread of metacercariae of F. hepatica. Secondly, the yaks in Gongbo’gyamda and Mainling regions are on free grazing, which increases the spread of F. hepatica infection.

The seroprevalence of F. hepatica increased from 20.75%-48.8% with age of yaks, with the further distribution of 20.75%, 30.23%, 39.55%, 37.70% and 48.8% for age groups: <0.5 year, 0.5-1 year, >1-2 year, >2-4 years and >4 years, respectively. The seroprevalence in male and female yaks was 32.51% and 39.05%, respectively (Table I). Yaks are the production partner and source of wealth for Tibetans. Female yaks (especially for old female yaks) were used for breeding, that will not be killed. So, female yaks have many chances to have greater exposure risks than male yaks. In present study, the overall seroprevalence of F. hepatica was 35.92%, which was higher than that reported in yaks in Gansu (28.7%) , cattle (28.6%) and goats (26.0%) in Yunnan, China , and buffaloes in Brazil (3%) ; while, lower than the seroprevalence in buffaloes (44.7%) in Hunan, China . Meanwhile, three mainly pre-identified probable risk factors including regions, age and gender were evaluated. The results showed that the seroprevalence of F. hepatica in females (39.05%; 95% CI 34.5%-43.8%) was 1.33 times (OR=1.33) more than male (32.51%; 95% CI 28.0%-37.3%). The gender based results have no statistically significant to influence the seroprevalence of F. hepatica among yaks, it has also suggested that the incidence of F. hepatica case have no relationship with exact gender. Odds ratio showed that yaks lived in Gongbo’gyamda (OR=1.99) and Mainling (OR=1.87) has 1.99 and 1.87-fold higher risk of F. hepatica infection as compared with yaks lived in Bayi (24.20%), respectively. Moreover, the results found the seroprevalence of F. hepatica in yaks of >4 years, >2-4 years, >1-2 year, and 0.5-1 year was 3.64, 2.31, 2.50, and 1.65 fold (OR=3.23, 95% CI=5.8-14.9) higher risk than yaks of <0.5 year, respectively. The seroprevalence and risk of F. hepatica in yaks increase with age as well, which was in accordance with some previous reports of cumulative effect with the age in the seroprevalence of F. hepatica in ruminants ; ; . The present study suggests that adult yaks have more chances to expose with F. hepatica through free-range outside the pasturing area, while calves’ yaks (<0.5 years of age) have less chances to encounter with metacercariae of F. hepatica. In our study, the physical examination showed a poor body condition with emaciation, weakness, loss of appetite, anemia, jaundice, and abdominal edema in infected yaks. After dissection, many prominent nodules of parasitic migration can be seen on the liver surface, and many yaks were found infested with F. hepatica in liver revealing the high infection rate in yaks. Our physical examination and pathological findings concomitant with the previous finding of Mehmood et al. (2017) and Wang et al. (2006).

Our study on the prevalence of F. hepatica provides a guideline in clinical medication, and it will also help to build important strategies and maintain a strong surveillance program to control and monitor the emerging F. hepatica infection in yaks and other food animals in Tibet.

Acknowledgements

The study was supported by research and demonstration of technologies for prevention and control of major infectious diseases in characteristic livestock (XZ201901NA02), key laboratory of clinical veterinary medicine in Tibet and Tibet Autonomous Region Science Fund (XZ2017ZRG-27; XZ2017ZRG-19Z).

Table I. Seroprevalence of F. hepatica in Yaks in different areas, ages, and genders on Tibetan Plateau, China.

Categories

Variables

Positive/examined

Prevalence (%)

OR value

95% CI

Regions

Bayi

38/157

24.20%

Ref

17.7%-31.7%

Mainling

92/242

38.02%

1.87

31.9%-44.5%

Gongbo'gyamda

175/450

38.89%

1.99

34.4%-43.6%

Ages

<0.5 year

22/106

20.75%

Ref

13.5%-29.7%

0.5-1 year

65/215

30.23%

1.65

24.2%-36.8%

>1-2 year

87/220

39.55%

2.50

33.0%-46.3%

>2-4 years

69/183

37.70%

2.31

30.7%-45.2%

>4 years

61/125

48.80%

3.64

39.8%-57.9%

Genders

Male

132/406

32.51%

Ref

28.0%-37.3%

Female

173/443

39.05%

1.33

34.5%-43.8%

Total

305/849

35.92%

32.7%-39.3%

Supplementary material

There is supplementary material associated with this article. Access the material online at: https://dx.doi.org/10.17582/journal.pjz/20190823220816

Statement of conflict of interest

The authors declare no conflict of interest.

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